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| Product Name | Sphingomyelin Colorimetric Assay Kit |
| Catalog No. | BLTR-0023 |
| Description | This sphingomyelin colorimetric assay kit uses enzymatic colorimetric method to detect sphingomyelin accurately. It has a suitable linear range, good precision, and is suitable for studying sphingomyelin - related cell signaling and metabolic pathways |
| Test Target | Sphingomyelin |
| Methodology | Enzymatic colorimetric (sphingomyelinase, choline oxidase, peroxidase) |
| Measurement Range | 0.03-3 mmol/L |
| Sample Type | Serum, plasma, cell membranes, tissue homogenates |
| Assay Time | 20 min at 37°C |
| Wavelength | 505 nm |
| Linearity | 0.03-3 mmol/L |
| Precision (Intra-assay CV) | ≤2.5% |
| Precision (Inter-assay CV) | ≤3.5% |
| Stability (Unopened) | 18 months at 2-8°C |
| Stability (Opened) | 28 days at 2-8°C |
| Stability (Reconstituted) | 10 days at 2-8°C |
| Interference (Hemoglobin) | ≤500 mg/dL (no significant interference) |
| Interference (Bilirubin) | ≤20 mg/dL (no significant interference) |
| Interference (Lipemic) | ≤1000 mg/dL (no significant interference) |
| Reagent Composition | Sphingomyelinase, choline oxidase, peroxidase, 4 - aminoantipyrine, phenol |
| Reagent Form | Liquid |
| Reagent Volume | R1: 40 mL; R2: 10 mL; Substrate: 5 mL |
| Calibration Type | External calibration |
| Calibration Levels | 3 levels |
| Incubation Temperature | 37°C |
| Detection Instrument | Spectrophotometer, microplate reader |
| Application | Quantitative determination of sphingomyelin in biological samples for research on cell signaling, lipid metabolism and neurodegenerative diseases |
| Cross - Reactivity | No significant cross - reactivity with other phospholipids |
| pH Range | 7.0-7.4 |
| Shelf Life | 18 months |
| Storage Condition | Store at 2-8°C |
For research use only, not for clinical use.
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