- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | SphI Rapid Restriction Endonuclease (20 U/uL) |
| Catalog No. | IVDRM-0466 |
| Description | This rapid recombinant endonuclease cuts 5 prime GCATG^C 3 prime to leave a 3 prime overhang and digests plasmid DNA, PCR products and genomic DNA within 5 to 15 minutes. Supplied at 20 U/uL, it runs in the shared rapid series buffer at 37°C and is heat inactivated at 80°C for 20 minutes. EcoBI methylation possibly overlapping the site may block cleavage, and the isoschizomers include BbuI, PaeI and SpaHI. |
| Intended Use | Rapid digestion of plasmid DNA, PCR products and genomic DNA for molecular cloning |
| Principle / Technology | Site specific cleavage at 5 prime GCATG^C 3 prime within 5 to 15 minutes at 37°C |
| Specificity | EcoBI methylation may block cleavage; isoschizomers include BbuI, PaeI and SpaHI |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 50 T |
| Key Features | 3 prime overhang output; one shared buffer for the whole rapid series |
| Concentration | 20 U/uL |
| Readouts / Downstream Assays | Agarose gel analysis of digested DNA |
For in vitro diagnostic use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
