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| Product Name | SpeI Rapid Restriction Endonuclease (20 U/uL) |
| Catalog No. | IVDRM-0465 |
| Description | Recognising 5 prime A^CTAGT 3 prime, this rapid recombinant endonuclease generates cohesive ends compatible with NheI and XbaI digests, a property widely exploited in assembly cloning and multi fragment construction. It digests plasmid DNA, PCR products and genomic DNA within 5 to 15 minutes, is supplied at 20 U/uL, and operates in the shared rapid series buffer at 37°C with heat inactivation at 80°C for 20 minutes. EcoKI and EcoBI methylation possibly overlapping the site may affect cutting, and the isoschizomers AhlI and BcuI share the specificity. |
| Intended Use | DNA fragment preparation with compatible cohesive ends for assembly cloning and molecular cloning |
| Principle / Technology | Site specific cleavage at 5 prime A^CTAGT 3 prime within 5 to 15 minutes at 37°C |
| Specificity | EcoKI and EcoBI methylation may affect cleavage; isoschizomers AhlI and BcuI |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 50 T |
| Key Features | Cohesive ends compatible with NheI and XbaI; single shared buffer across the rapid series |
| Concentration | 20 U/uL |
| Readouts / Downstream Assays | Restriction digest pattern on agarose gel |
For in vitro diagnostic use.
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