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| Product Name | Sodium Pyruvate, 100 mM Solution, 100x |
| Catalog No. | ATR-CCR-0012 |
| Description | A sterile 100 mM sodium pyruvate solution at 100x concentration, providing an additional energy substrate for cell culture through its role as an intermediate in the glycolytic pathway and Krebs cycle. |
| Intended Use | Supplementing cell culture media with pyruvate to enhance cell energy metabolism, reduce lactic acid accumulation, and improve growth of cell lines with high glycolytic activity or impaired mitochondrial function. |
| Principle / Technology | Sodium pyruvate enters the Krebs cycle as acetyl-CoA or can be reduced to lactate, serving as an alternative energy source that spares glucose; it also functions as an antioxidant by scavenging hydrogen peroxide. |
| Detection Method | Cell growth rate, lactate production measurement, viability under oxidative stress |
| Sample Type | Culture media for mammalian cell lines, particularly those with high energy demands or cultured at high density |
| Performance Range / Specifications | 1 mM final concentration at 1:100 dilution; 15–25% reduction in culture medium lactate accumulation |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Add 5 mL per 500 mL medium (1:100 dilution); stable in complete medium for the duration of medium shelf life. |
| Components / Formulation | Sodium pyruvate 100 mM in cell culture grade water, sterile-filtered |
| Storage Conditions | 2–8°C, protect from light |
| Shelf Life | 24 months |
| Package Specifications | 100 mL, 500 mL |
| Product Form | Liquid concentrate (100x), sterile |
| Quality Control | Concentration verification, sterility, endotoxin, cell metabolism enhancement validation |
| Key Features | Sterile 100x concentrate; energy substrate supplement; reduces lactate build-up; antioxidant properties; ready to use |
For research use only, not for clinical use.
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