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| Product Name | SmaI Rapid Restriction Endonuclease (20 U/uL) |
| Catalog No. | IVDRM-0464 |
| Description | This rapid recombinant endonuclease cuts the GC rich site 5 prime CCC^GGG 3 prime to give blunt ends and digests plasmid DNA, PCR products and genomic DNA within 5 to 15 minutes. Unusually for the series, its optimal incubation temperature is 25°C rather than 37°C, and it operates in the shared rapid buffer with heat inactivation afterwards at 80°C for 20 minutes. CpG methylation fully overlapping the site blocks cleavage, and no alternative named isoschizomers are documented. |
| Intended Use | Rapid blunt ended digestion for DNA fragment preparation in molecular cloning |
| Principle / Technology | Site specific blunt cleavage at 5 prime CCC^GGG 3 prime within 5 to 15 minutes at 25°C optimum |
| Specificity | CpG methylation fully overlapping the site blocks cleavage; no alternative named isoschizomers |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer with 25°C incubation for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 100 T |
| Key Features | Blunt end output at a low temperature optimum; single shared buffer across the rapid series |
| Concentration | 20 U/uL |
| Readouts / Downstream Assays | Gel electrophoresis of digested DNA |
For in vitro diagnostic use.
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