Shrimp Alkaline Phosphatase, Heat-Labile (SAP, 1 U/uL)

Name: Shrimp Alkaline Phosphatase, Heat-Labile (SAP, 1 U/uL)
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Cat.No: IVDRM-0458 Datasheet

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Product Name	Shrimp Alkaline Phosphatase, Heat-Labile (SAP, 1 U/uL)
Catalog No.	IVDRM-0458
Description	Expressed in Pichia pastoris from the Arctic shrimp Pandalus alkaline phosphatase gene, this enzyme removes 5 prime phosphate groups from DNA and RNA ends, whether cohesive or blunt, and hydrolyses nearly all phosphomonoesters without attacking phosphodiester or phosphotriester bonds. Its defining advantage is rapid heat lability: 5 minutes at 65°C irreversibly abolishes activity, letting dephosphorylation and subsequent steps proceed in the same tube. Beyond preventing vector self ligation and preparing 5 prime end labeling templates, SAP degrades residual dNTPs in PCR products to ready them for sequencing and SNP analysis. The preparation exceeds 95% purity and is verified free of RNase and DNase contamination.
Intended Use	Vector dephosphorylation, 5 prime end labeling template preparation and dNTP degradation for sequencing and SNP analysis
Principle / Technology	Terminal dephosphorylation of cohesive and blunt DNA and RNA ends
Reaction Conditions / Protocol	Heat inactivate at 65°C for 5 minutes for irreversible loss of activity
Storage Conditions	-25 to -15°C
Shelf Life	3 years
Package Specifications	250 U
Quality Control	Free of RNase and DNase contamination; unit defined by dephosphorylation of 1 ug HindIII digested pUC19 in 30 minutes at 25°C
Key Features	Fast heat lability enables same tube workflows; blunt and cohesive end substrates both accepted
Purity	Greater than 95%
Concentration	1 U/uL
Source / Origin	Arctic shrimp Pandalus alkaline phosphatase gene expressed in Pichia pastoris
Readouts / Downstream Assays	Dephosphorylation efficiency and post-treatment sequencing or SNP assay performance

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For in vitro diagnostic use.