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| Product Name | SfiI Rapid Restriction Endonuclease (20 U/uL) |
| Catalog No. | IVDRM-0470 |
| Description | Recognising the bipartite asymmetric site 5 prime GGCCNNNN^NGGCC 3 prime, this rapid recombinant endonuclease supports plasmid linearisation, vector construction and DNA fragment cloning, digesting plasmid DNA, PCR products and genomic DNA within 5 to 15 minutes. Unusually for the series its optimal reaction temperature is 50°C Supplied at 20 U/uL, it runs in the shared rapid buffer. Dcm and CpG methylation affect cleavage at overlapping sequences, and no alternative named isoschizomers are documented. Note that the enzyme cannot be heat inactivated; remove it after digestion by phenol chloroform extraction or column purification. |
| Intended Use | Plasmid linearisation, vector construction and DNA fragment cloning with bipartite site digestion |
| Principle / Technology | Bipartite site specific cleavage at 5 prime GGCCNNNN^NGGCC 3 prime within 5 to 15 minutes at 50°C optimum |
| Specificity | Dcm and CpG methylation affect cleavage; no alternative named isoschizomers |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer with 50°C incubation for 5 to 15 minutes; not heat inactivatable, remove enzyme by phenol chloroform extraction or column purification |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 100 T |
| Key Features | Bipartite recognition with long spacer enables directional cloning designs; high temperature optimum |
| Concentration | 20 U/uL |
| Readouts / Downstream Assays | Restriction digest pattern on agarose gel |
For in vitro diagnostic use.
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