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| Product Name | Serum-Free, Xeno-Free Expansion Medium for Human Mesenchymal Stem Cells, Plus |
| Catalog No. | RERE-0020 |
| Description | This medium supports the expansion of human mesenchymal-type stem cells from umbilical cord, bone marrow, adipose tissue and cord blood sources in a serum-free, xeno-free system. Compared with traditional serum-containing formulations, it keeps the cells in undifferentiated growth, preserves their characteristic spindle shape and normal chromosome karyotype, and sustains a high proliferation rate along with trilineage differentiation potential - into osteogenic, adipogenic and chondrogenic lineages. A serum replacement is pre-added, so the bottle opens ready to use, which also lowers the risk of cross-contamination during preparation. It is prepared with water for injection. |
| Intended Use | Serum-free, xeno-free expansion of human mesenchymal stem cells from umbilical cord, bone marrow, adipose or cord blood sources, while preserving morphology, karyotype and trilineage differentiation potential. |
| Principle / Technology | A fully defined, xeno-free formulation replaces animal serum and keeps MSCs in undifferentiated expansion; the pre-added serum replacement makes the medium ready to use straight from the bottle. |
| Detection Method | Culture performance is judged by standard MSC read-outs - spindle morphology, surface marker flow cytometry, karyotype and trilineage differentiation staining run by the laboratory. |
| Sample Type | Human mesenchymal stem cells from umbilical cord, bone marrow, adipose tissue or cord blood. |
| Performance Range / Specifications | Serum-free; xeno-free; pre-added serum replacement, open-and-use; maintains spindle morphology and normal karyotype; high proliferation with preserved trilineage (osteogenic, adipogenic, chondrogenic) potential; prepared with water for injection; 500 mL bottle. |
| Specificity | Formulated for human mesenchymal-type stem cells across multiple tissue sources; trilineage differentiation staining reagents are available separately. |
| Reaction Conditions / Protocol | Equilibrate the medium, culture the MSCs with standard feeding schedules, monitor morphology and expansion, and confirm differentiation potential with the osteogenic, adipogenic and chondrogenic staining workflows when required. |
| Components / Formulation | One 500 mL bottle of complete medium with pre-added serum replacement. |
| Storage Conditions | Store at -25 to -15 °C, protected from light. |
| Shelf Life | 12 months under the recommended storage conditions. |
| Package Specifications | 500 mL per bottle |
| Product Form | Liquid complete medium. |
| Quality Control | Lot release covers the maintenance of morphology, karyotype stability and differentiation potential during expansion. |
| Key Features | - Serum-free and xeno-free for clean MSC expansion - Pre-added serum replacement - open the bottle and use - Preserves spindle morphology and normal karyotype - Holds trilineage differentiation potential through expansion - Works across cord, bone marrow, adipose and cord blood sources |
| Concentration | Supplied as a ready-to-use complete medium. |
| Activity / Unit Definition | One bottle supports routine MSC expansion; performance is defined by the morphology, karyotype and trilineage outcomes. |
| Source / Origin | Manufactured from defined raw materials, prepared with water for injection; xeno-free. |
| Shipping Conditions | Shipped frozen on dry ice. |
| Expiration Date / Stability | Stability is maintained by the recommended storage temperature together with continuous protection from light. |
| Regulatory / Compliance | Supplied for laboratory research purposes. |
| Compatibility | Complements the separately available trilineage staining reagents: Alizarin Red S for osteogenesis, Oil Red O for adipogenesis and AB-PAS for chondrogenesis. |
| Recommended Buffer System | Supplied complete and ready to use; no separate supplement dilution is required. |
| Application Notes / Precautions | Thaw completely and mix gently before use, avoid repeated freeze-thaw cycles by aliquoting if needed, and confirm the differentiation potential at defined passage windows rather than assuming it carries over indefinitely. |
| Batch-to-Batch Consistency | Each production lot is released against fixed acceptance criteria, keeping performance consistent between orders. |
| Model Type | Serum-free xeno-free cell culture medium (complete formulation) |
| Starting Material / Tissue Site | Defined basal formulation with pre-added serum replacement, prepared with water for injection |
| Delivery Standard | 500 mL bottle |
| Turnaround Time | dispatch timing is settled at purchase. |
| Readouts / Downstream Assays | MSC morphology, karyotype and trilineage staining run by the user laboratory. |
For research use only, not for clinical use.
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