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| Product Name | Serum-Free Medium (for Exosome Production) |
| Catalog No. | RESK-0615 |
| Description | Exosomes are small vesicles approximately 30-150 nm in diameter secreted by living cells, with a typical lipid bilayer structure. They are present in cell culture supernatant, serum, plasma, saliva, urine, amniotic fluid, and other biological fluids. Exosomes carry important cargo such as proteins, lipids, DNA, and RNA; they not only play important roles in intercellular material and information transfer, but are also promising early diagnostic biomarkers for a variety of diseases. This product is a medium developed specifically for exosome production. Its main components are HEPES, L-glutamine, pharmaceutical-grade albumin, hypoxanthine, thymidine, phenol red, and glucose. It is xeno-free and serum-free and contains no growth factors. During cell culture, no serum supplementation is required to reach the culture state of complete medium, which facilitates exosome harvest. |
| Intended Use | Serum-free culture of cells (tumor cell lines and T cells) for exosome production and harvest of exosome-containing cell culture supernatant. For research use only. |
| Principle / Technology | A serum-free, xeno-free formulation without growth factors supports cell culture to the state achieved with complete medium without serum supplementation, so that the harvested supernatant is free of serum-derived exosomes and well suited for exosome isolation. |
| Sample Type | Cultured cells: tumor cell lines and T cells (adherent culture). |
| Performance Range / Specifications | Suitable cells: tumor cell lines and T cells. Recommended medium volumes: 3 mL for a 6-well plate, 5 mL for a 60 mm dish, 10 mL for a 100 mm dish, 15 mL for a 150 mm dish. Validation data (HeLa cells): colony formation comparison between complete medium and this serum-free medium; electron microscopy identification of exosomes from exosome-depleted serum, normal serum, and this serum-free medium culture groups; NTA analysis of harvested exosomes. |
| Reaction Conditions / Protocol | 1. After cell recovery, culture cells in complete medium for 2-3 passages until the cells reach a normal growth state. 2. Gradient adaptation step 1: when cells reach approximately 50% confluence, remove the complete medium and add fresh medium consisting of 50% serum-free medium for exosome production and 50% complete medium. 3. Gradient adaptation step 2: when cells in step 1 reach approximately 70-80% confluence, remove the 50%/50% medium mixture. 4. Rinse the cells 2-3 times with 1×PBS. 5. Add the serum-free medium for exosome production and continue culture for 24-48 h; when cells reach 90-100% confluence, collect the cell culture supernatant, which can then be used for exosome extraction experiments. |
| Components / Formulation | Main components: HEPES, L-glutamine, pharmaceutical-grade albumin, hypoxanthine, thymidine, phenol red, and glucose. Xeno-free, serum-free, and free of growth factors; contains no antibiotics and no platelet lysate. |
| Storage Conditions | Store at 2-8 °C, protected from light. |
| Shelf Life | 1 year when stored as recommended. |
| Package Specifications | 50 mL 500 mL |
| Product Form | Liquid medium (ready to use). |
| Shipping Conditions | Shipped with ice packs. |
| Expiration Date / Stability | Valid for 1 year when stored at 2-8 °C protected from light. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Designed for most tumor cell lines and T cells; not validated on endothelial cells; not suitable for THP-1 cells (monocytic suspension cells). This medium cannot be used for cell passaging and is intended only for collecting cell culture supernatant. |
| Recommended Buffer System | 1×PBS is used to rinse cells before switching to the serum-free medium. |
| Application Notes / Precautions | The medium contains no antibiotics; antibiotics (e.g., 1% penicillin-streptomycin) can be added if needed. This medium cannot be used for cell passaging; it is used only for collecting cell culture supernatant for exosome extraction. This medium has not been validated on endothelial cells; it is designed for most tumor cells, and testing a small package size first is recommended. THP-1 cells are monocytic suspension cells and are not suitable for this medium. The product does not contain platelet lysate. |
For research use only, not for clinical use.
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