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| Product Name | Serum-Free Cell Freezing Medium, Phenol Red-Free |
| Catalog No. | RERE-0220 |
| Description | Chemically defined, serum-free freezing medium for mammalian cell cryopreservation, formulated with carbohydrates, amino acids and DMSO-based cryoprotectants and without phenol red. Because the composition is defined, the formulation avoids the batch variability and adventitious-agent concerns of serum-containing freeze mixes, and no programmed cooling is required: cells resuspended in the medium go directly to -80°C and transfer to liquid nitrogen the next day. Suited to routine mammalian lines including tumour, transformed, primary, stem and immune cells, particularly cultures maintained in serum-free systems; the phenol red-free base also fits freezing cells destined for hormone-related experiments. At 1 mL per cryovial, a 50 mL bottle covers roughly 50 vials and a 100 mL bottle roughly 100. Unopened bottles keep for 12 months at 2-8°C. |
| Intended Use | Cryopreservation of mammalian lines without serum or programmed cooling, including serum-free cultures and hormone-experiment cells |
| Principle / Technology | Defined cryoprotectant matrix lowers freezing point and draws intracellular water out before ice forms, limiting crystal damage without serum or rate-controlled freezing |
| Specificity | Sterile solution; no phenol red; chemically defined, serum-free composition; 12 month shelf life |
| Reaction Conditions / Protocol | Resuspend the cell pellet directly in chilled medium, aliquot 1 mL per cryovial and place at -80°C, then transfer to liquid nitrogen the following day |
| Storage Conditions | 2-8°C |
| Package Specifications | 50 mL | 100 mL |
| Key Features | Serum-free and phenol red-free; no programmed cooling required; defined composition; 12 month shelf life |
For research use only, not for clinical use.
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