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| Product Name | SdaI (SbfI) Rapid Restriction Endonuclease (20 U/uL) |
| Catalog No. | IVDRM-0471 |
| Description | Cutting the eight base site 5 prime CCTGCA^GG 3 prime, this rapid recombinant endonuclease processes plasmid DNA, PCR products and genomic DNA for molecular cloning and finishes digestion within 5 to 15 minutes. It is supplied at 20 U/uL, works in the unified rapid series buffer at 37°C and is heat inactivated at 80°C for 20 minutes. Dam, Dcm, CpG, EcoKI and EcoBI methylation leave cutting unaffected, and SbfI together with Sse8387I share the same specificity. |
| Intended Use | Rapid digestion of plasmid DNA, PCR products and genomic DNA for molecular cloning |
| Principle / Technology | Site specific cleavage at 5 prime CCTGCA^GG 3 prime within 5 to 15 minutes at 37°C |
| Sample Type | Plasmid DNA; PCR products; genomic DNA |
| Specificity | Unaffected by Dam, Dcm, CpG, EcoKI and EcoBI methylation; isoschizomers SbfI and Sse8387I |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 25 T |
| Key Features | Single shared buffer across the rapid series; activity redundancy for excess substrate |
| Concentration | 20 U/uL |
| Readouts / Downstream Assays | Agarose gel profile of digested DNA |
For in vitro diagnostic use.
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