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| Product Name | Salt-Tolerant T4 DNA Ligase for High-Salt Ligation Conditions (400 U/uL) |
| Catalog No. | IVDRM-0344 |
| Description | This mutant of T4 DNA ligase catalyses phosphodiester bond formation between adjacent 5′ phosphate and 3′ hydroxyl termini in double-stranded DNA or RNA, with ATP as cofactor. Compared with the wild-type enzyme it tolerates salt concentrations up to 300 mM, and it joins blunt and cohesive ends while also repairing single-strand nicks in double-stranded DNA and some DNA/RNA hybrids. |
| Intended Use | Adapter ligation during NGS library construction; molecular cloning with blunt or cohesive ends; nick repair in double-stranded DNA and DNA/RNA hybrids. |
| Specificity | Salt tolerance up to 300 mM; ATP-dependent activity |
| Storage Conditions | -25 °C to -15 °C |
| Shelf Life | 24 months |
| Package Specifications | 20 KU and 100 KU packs |
| Purity | ≥95% |
| Concentration | 400 U/uL |
| Source / Origin | Salt-tolerant mutant of T4 DNA ligase, recombinant expression in E. coli |
| Recommended Buffer System | 10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% glycerol, pH 7.4 at 25 °C |
| Model Type | Salt-tolerant T4 DNA ligase mutant |
For in vitro diagnostic use.
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