SalI Rapid Restriction Endonuclease (20 U/uL)
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SalI Rapid Restriction Endonuclease (20 U/uL)

Cat.No: IVDRM-0462 Datasheet

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Product Details Related Products
Product Name SalI Rapid Restriction Endonuclease (20 U/uL)
Catalog No. IVDRM-0462
Description Cutting 5 prime G^TCGAC 3 prime to generate cohesive ends compatible with XhoI overhangs, this rapid recombinant endonuclease is a mainstay of cloning cassette design and general molecular cloning. It digests plasmid DNA, PCR products and genomic DNA within 5 to 15 minutes, is supplied at 20 U/uL, and operates in the common rapid series buffer at 37°C with heat inactivation at 80°C for 20 minutes. CpG methylation fully overlapping the site blocks cleavage, and no alternative named isoschizomers are documented.
Intended Use Molecular cloning and cloning cassette design with rapid digestion
Principle / Technology Site specific cleavage at 5 prime G^TCGAC 3 prime within 5 to 15 minutes at 37°C
Specificity CpG methylation fully overlapping the site blocks cleavage; no alternative named isoschizomers
Reaction Conditions / Protocol Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes
Storage Conditions -25 to -15°C
Shelf Life 2 years
Package Specifications 200 T
Key Features Large 200 reaction pack; overhang compatible with XhoI digests for flexible cloning
Concentration 20 U/uL
Readouts / Downstream Assays Agarose gel analysis of digested DNA

For in vitro diagnostic use.

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