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| Product Name | SalI Rapid Restriction Endonuclease (20 U/uL) |
| Catalog No. | IVDRM-0462 |
| Description | Cutting 5 prime G^TCGAC 3 prime to generate cohesive ends compatible with XhoI overhangs, this rapid recombinant endonuclease is a mainstay of cloning cassette design and general molecular cloning. It digests plasmid DNA, PCR products and genomic DNA within 5 to 15 minutes, is supplied at 20 U/uL, and operates in the common rapid series buffer at 37°C with heat inactivation at 80°C for 20 minutes. CpG methylation fully overlapping the site blocks cleavage, and no alternative named isoschizomers are documented. |
| Intended Use | Molecular cloning and cloning cassette design with rapid digestion |
| Principle / Technology | Site specific cleavage at 5 prime G^TCGAC 3 prime within 5 to 15 minutes at 37°C |
| Specificity | CpG methylation fully overlapping the site blocks cleavage; no alternative named isoschizomers |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 200 T |
| Key Features | Large 200 reaction pack; overhang compatible with XhoI digests for flexible cloning |
| Concentration | 20 U/uL |
| Readouts / Downstream Assays | Agarose gel analysis of digested DNA |
For in vitro diagnostic use.
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