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| Product Name | SacII Rapid Restriction Endonuclease (20 U/uL) |
| Catalog No. | IVDRM-0469 |
| Description | Cutting the CpG rich site 5 prime CCGC^GG 3 prime, this rapid recombinant endonuclease supports vector construction and DNA fragment preparation with digestion of plasmid DNA, PCR products and genomic DNA in 5 to 15 minutes. CpG methylation fully overlapping the site blocks cleavage, an essential consideration for methylated templates. Supplied at 20 U/uL, it runs in the shared rapid series buffer at 37°C and is heat inactivated at 80°C for 20 minutes. This unit stores at -20°C and ships with ice packs. |
| Intended Use | Vector construction and DNA fragment preparation with rapid digestion |
| Principle / Technology | Site specific cleavage at 5 prime CCGC^GG 3 prime within 5 to 15 minutes at 37°C |
| Specificity | CpG methylation fully overlapping the site blocks cleavage |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -20°C |
| Shelf Life | 2 years |
| Package Specifications | 50 T |
| Key Features | Single shared buffer across the rapid series; activity redundancy for difficult templates |
| Concentration | 20 U/uL |
| Shipping Conditions | Ice packs |
| Readouts / Downstream Assays | Agarose gel analysis of digested DNA |
For in vitro diagnostic use.
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