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| Product Name | RsaI Rapid Restriction Endonuclease (10 U/uL) |
| Catalog No. | IVDRM-0459 |
| Description | Recognising the tetranucleotide site 5 prime GT^AC 3 prime, this rapid recombinant endonuclease prepares DNA fragments for cloning and supports digest verification and restriction map analysis, cutting plasmid DNA, PCR products and genomic DNA within 5 to 15 minutes. Supplied at 10 U/uL, it runs in the shared rapid series buffer at 37°C Dam, Dcm, EcoKI and EcoBI methylation leave activity unchanged while CpG methylation at overlapping sites affects cutting, and the isoschizomers include AfaI, Csp6I, CviQI and RsaNI. Note that the enzyme cannot be heat inactivated; use phenol chloroform extraction or column purification for removal. |
| Intended Use | DNA fragment preparation for cloning and restriction mapping verification |
| Principle / Technology | Site specific cleavage at 5 prime GT^AC 3 prime within 5 to 15 minutes at 37°C |
| Specificity | Dam, Dcm, EcoKI and EcoBI methylation have no effect; CpG methylation affects cleavage; isoschizomers include AfaI, Csp6I, CviQI and RsaNI |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; not heat inactivatable, remove enzyme by phenol chloroform extraction or column purification |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 100 T |
| Key Features | Frequent tetranucleotide cutter; suited to double and multiplex digestion in the shared buffer |
| Concentration | 10 U/uL |
| Readouts / Downstream Assays | Restriction map pattern on agarose gel |
For in vitro diagnostic use.
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