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| Product Name | RPMI-1640 Medium, with L-Glutamine, 25 mM HEPES |
| Catalog No. | ATR-CCR-0002 |
| Description | Roswell Park Memorial Institute 1640 medium buffered with HEPES, originally developed for the culture of human leukocytes and widely used for lymphoid cell lines, hybridomas, and primary human lymphocytes. |
| Intended Use | Culture of human lymphoid cells, hybridoma lines for monoclonal antibody production, peripheral blood mononuclear cells, and leukemia/lymphoma cell lines in suspension culture. |
| Principle / Technology | Formulated with HEPES organic buffer providing additional pH stability in ambient CO2 conditions or during cell handling outside the incubator; bicarbonate buffer system maintains pH in 5% CO2 atmosphere. |
| Detection Method | Cell density and viability by trypan blue exclusion, immunoglobulin secretion by ELISA for hybridomas |
| Sample Type | Human PBMCs, hybridoma cells, Jurkat, Raji, HL-60, K562, and other hematopoietic cell lines |
| Performance Range / Specifications | Supports cell densities up to 2 × 10^6 cells/mL for suspension lymphoid lines; doubling times 20–30 hours |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Supplement with 10–20% FBS, 1% penicillin-streptomycin, and 50 µM 2-mercaptoethanol for lymphocyte culture; incubate in 5% CO2 at 37°C. |
| Components / Formulation | RPMI-1640 basal salts, amino acids, vitamins, 2 g/L glucose, 2 g/L sodium bicarbonate, 25 mM HEPES, 2 mM L-glutamine, phenol red |
| Storage Conditions | 2–8°C, protect from light |
| Shelf Life | 12 months |
| Package Specifications | 500 mL, 1 L |
| Product Form | Liquid, sterile-filtered |
| Quality Control | Sterility, endotoxin <0.25 EU/mL, pH, osmolality, cell growth promotion |
| Key Features | HEPES buffered for pH stability; standard for lymphoid cells; with L-glutamine; validated for hybridoma and PBMC culture |
For research use only, not for clinical use.
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