- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | RPMI 1640 Medium (with HEPES, L-Glutamine, D-Glucose and Phenol Red; without Sodium Pyruvate) |
| Catalog No. | RESK-0637 |
| Description | RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium) was originally developed for the suspension or monolayer culture of human leukemia cells and has since proven suitable for a wide range of mammalian cells, including HeLa, Jurkat, MCF-7, PC-12, PBMCs, astrocytes and carcinoma cells. It is particularly well suited for suspension cell culture and is one of the most widely used cell culture media. RPMI 1640 is distinguished from other media by its content of reduced glutathione and high vitamin concentrations. It contains biotin, vitamin B12 and para-aminobenzoic acid, which are absent from EMEM and DMEM, as well as high concentrations of choline chloride and inositol. This product is prepared with water for injection and sterilized by 0.1 um filtration. Contains: HEPES, L-glutamine, D-glucose and phenol red. Without: sodium pyruvate. |
| Intended Use | For in vitro culture of a broad range of mammalian cells, including HeLa, Jurkat, MCF-7, PC-12, PBMCs, astrocytes and carcinoma cells; especially suitable for suspension cell culture. |
| Performance Range / Specifications | Complete formulation with HEPES, L-glutamine, D-glucose and phenol red; without sodium pyruvate. Sterilized by 0.1 um filtration. |
| Components / Formulation | Amino Acids Glycine 10 mg/L L-Arginine 200 mg/L L-Asparagine 50 mg/L L-Aspartic acid 20 mg/L L-Cystine 2HCl 65 mg/L L-Glutamic Acid 20 mg/L L-Glutamine 300 mg/L L-Histidine 15 mg/L L-Hydroxyproline 20 mg/L L-Isoleucine 50 mg/L L-Leucine 50 mg/L L-Lysine hydrochloride 40 mg/L L-Methionine 15 mg/L L-Phenylalanine 15 mg/L L-Proline 20 mg/L L-Serine 30 mg/L L-Threonine 20 mg/L L-Tryptophan 5 mg/L L-Tyrosine disodium salt dihydrate 29 mg/L L-Valine 20 mg/L Vitamins Biotin 0.2 mg/L Choline chloride 3 mg/L D-Calcium pantothenate 0.25 mg/L Folic Acid 1 mg/L Niacinamide 1 mg/L Para-Aminobenzoic Acid 1 mg/L Pyridoxine hydrochloride 1 mg/L Riboflavin 0.2 mg/L Thiamine hydrochloride 1 mg/L Vitamin B12 0.005 mg/L i-Inositol 35 mg/L Inorganic Salts Calcium nitrate (Ca(NO3)2 4H2O) 100 mg/L Magnesium Sulfate (MgSO4, anhydrous) 48.84 mg/L Potassium Chloride (KCl) 400 mg/L Sodium Bicarbonate (NaHCO3) 2000 mg/L Sodium Chloride (NaCl) 5300 mg/L Sodium Phosphate dibasic (Na2HPO4, anhydrous) 800 mg/L Other Components D-Glucose (Dextrose) 2000 mg/L Glutathione (reduced) 1 mg/L HEPES 5958 mg/L Phenol Red 5 mg/L |
| Storage Conditions | Store at 2-8 °C, protected from light. |
| Shelf Life | 1 year when stored at 2-8 °C, protected from light. |
| Package Specifications | 500 mL |
| Product Form | Liquid, 1x ready-to-use cell culture medium |
| Quality Control | Prepared with water for injection and sterilized by 0.1 um filtration. |
| Regulatory / Compliance | For research use only. |
| Application Notes / Precautions | This formulation contains HEPES and no sodium pyruvate; verify compatibility with the target cell line before use. Protect from light during storage. |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
