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| Product Name | RNase HII, Glycerol-Free (2 U/uL) |
| Catalog No. | IVDRM-0489 |
| Description | A ribonucleoside endonuclease from Pyrococcus abyssi produced by recombinant expression in E. coli, RNase HII recognises DNA-rN-DNA/DNA duplexes and cuts at the site where a ribonucleotide has been incorporated into DNA, while showing very low activity on single stranded RNA and none on single or double stranded DNA. Cleavage occurs from the 5 prime side of the ribonucleotide to leave a 5 prime phosphate and a 3 prime hydroxyl group. Activity is optimal between 70 and 75°C across a 50 to 75°C working range and is minimal at room temperature, and the enzyme tolerates 45 minutes at 95°C with almost no loss of activity. The glycerol-free formulation, stable at 2 to 8°C, suits lyophilised reaction systems. |
| Intended Use | LAMP probe assays, RNase HII dependent PCR and studies of ribonucleotide excision during DNA replication and repair |
| Principle / Technology | Endonucleolytic cleavage at ribonucleotide sites within DNA-rN-DNA/DNA duplexes, releasing 5 prime phosphate and 3 prime hydroxyl ends |
| Sample Type | DNA-rN-DNA/DNA duplexes; LAMP and rhPCR reaction mixes |
| Reaction Conditions / Protocol | Add to the reaction mix; activity is optimal at 70 to 75°C and the enzyme tolerates 95°C for 45 minutes |
| Storage Conditions | 2 to 8°C |
| Shelf Life | 6 months |
| Package Specifications | 1 KU; 10 KU |
| Key Features | Glycerol-free and suited to lyophilised systems; highly thermostable; host residue below 0.5 copies per U |
| Purity | Free of exonuclease, nickase and RNase residue |
| Concentration | 2 U/uL |
| Source / Origin | Pyrococcus abyssi, recombinant expression in E. coli |
| Recommended Buffer System | Glycerol-free formulation |
| Readouts / Downstream Assays | RNase HII dependent cleavage in LAMP or rhPCR assays |
For in vitro diagnostic use.
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