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| Product Name | RNase HII (2 U/uL) |
| Catalog No. | IVDRM-0498 |
| Description | A ribonucleoside endonuclease from Pyrococcus abyssi produced by recombinant expression in E. coli, RNase HII recognises DNA-rN-DNA/DNA duplexes and cuts at the site where a ribonucleotide is incorporated into DNA, while showing very low activity on single stranded RNA and none on single or double stranded DNA. Cleavage occurs from the 5 prime side of the ribonucleotide residue and leaves a 5 prime phosphate and a 3 prime hydroxyl group. Activity peaks between 70 and 75°C across a 50 to 75°C working range and is minimal at room temperature, and the enzyme tolerates 45 minutes at 95°C with almost no loss of activity, so it is compatible with PCR reaction systems. |
| Intended Use | LAMP probe assays, RNase HII dependent PCR, excision of misincorporated ribonucleotides and degradation of Okazaki fragment RNA |
| Principle / Technology | Endonucleolytic cleavage at ribonucleotide sites within DNA-rN-DNA/DNA duplexes, releasing 5 prime phosphate and 3 prime hydroxyl ends |
| Sample Type | DNA-rN-DNA/DNA duplexes; LAMP and rhPCR reaction mixes |
| Reaction Conditions / Protocol | Add to the reaction mix; activity is optimal at 70 to 75°C and the enzyme tolerates 95°C for 45 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 50 U; 250 U |
| Key Features | Highly thermostable; host genomic DNA below 0.5 copies per U; compatible with PCR systems |
| Purity | Free of exonuclease, nickase and RNase residue |
| Concentration | 2 U/uL |
| Source / Origin | Pyrococcus abyssi, recombinant expression in E. coli |
| Readouts / Downstream Assays | RNase HII dependent cleavage in LAMP or rhPCR assays |
For in vitro diagnostic use.
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