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| Product Name | RNase H for RNA Cleavage in Hybrid Duplexes (60 U/uL) |
| Catalog No. | IVDRM-0363 |
| Description | RNase H (ribonuclease H) is an endoribonuclease that specifically hydrolyses phosphodiester bonds of the RNA strand in DNA/RNA hybrid duplexes, and does not digest single-stranded or double-stranded DNA or RNA. Typical applications include removal of mRNA before second-strand cDNA synthesis, removal of RNA after first-strand synthesis in RT-PCR and RT-qPCR, removal of poly(A) tails after Oligo(dT) hybridisation to mRNA, and site-specific RNA cleavage. |
| Intended Use | mRNA removal before second-strand cDNA synthesis; RNA removal after first-strand cDNA in RT-PCR and RT-qPCR; poly(A) removal after Oligo(dT) hybridisation; site-specific RNA cleavage |
| Principle / Technology | Endoribonucleolytic cleavage of the RNA strand within DNA/RNA hybrids, leaving the DNA strand intact |
| Sample Type | RNA or cDNA synthesis reaction mixtures |
| Specificity | Hydrolyses RNA in DNA/RNA hybrid duplexes only; no activity on single-stranded or double-stranded DNA or RNA |
| Storage Conditions | -20°C |
| Shelf Life | 2 years |
| Package Specifications | 1 mL; 10 mL |
| Key Features | Specific to the RNA strand of hybrid duplexes; suited to NGS and mRNA library workflows |
| Concentration | 60 U/uL |
| Shipping Conditions | Dry ice |
For in vitro diagnostic use.
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