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| Product Name | RNase A (100 mg/mL) |
| Catalog No. | IVDRM-0549 |
| Description | A high concentration formulation of ribonuclease A, a single chain polypeptide of about 13.7 kDa carrying four disulfide bonds. As an endoribonuclease the enzyme specifically degrades cytosine and uracil residues on single stranded RNA, hydrolysing the phosphodiester bond between the 5 prime ribose of one nucleotide and the 3 prime phosphate of the adjacent pyrimidine, so the 2 prime, 3 prime cyclic phosphate resolves to the corresponding 3 prime nucleotide phosphate. Activity is highest on single stranded RNA and is salt dependent: at 0 to 100 mM sodium chloride the enzyme cuts single stranded RNA, double stranded RNA and the RNA strand of hybrids, while at 0.3 M and above it cuts single stranded RNA only. Supplied at 100 mg/mL in 50 mM Tris-HCl pH 7.4 with 50% glycerol, the recommended working range is 1 to 100 micrograms per millilitre, and one Kunitz unit is defined at 25°C and pH 5.0. |
| Intended Use | RNA removal during plasmid and genomic DNA preparation, RNA degradation for RNase protection and sequence analysis |
| Principle / Technology | Endoribonucleolytic cleavage after cytosine and uracil residues via a 2 prime, 3 prime cyclic phosphate intermediate |
| Sample Type | RNA contamination in DNA preparations |
| Specificity | Degrades C and U residues of single stranded RNA; working range 1 to 100 micrograms per millilitre |
| Reaction Conditions / Protocol | Treat the sample within the 1 to 100 micrograms per millilitre working range, then remove the enzyme |
| Storage Conditions | -20°C |
| Shelf Life | 2 years |
| Package Specifications | 1 mL |
| Key Features | High concentration format; Kunitz unit based activity defined at 25°C and pH 5.0 |
| Concentration | 100 mg/mL |
| Shipping Conditions | Ice pack shipping |
| Recommended Buffer System | 50 mM Tris-HCl pH 7.4 with 50% glycerol |
| Readouts / Downstream Assays | RNA clearance verified by gel electrophoresis |
For in vitro diagnostic use.
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