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| Product Name | RNase A (10 mg/mL) |
| Catalog No. | IVDRM-0548 |
| Description | Ribonuclease A is a single chain polypeptide of about 13.7 kDa with four disulfide bonds, and as an endoribonuclease it specifically degrades cytosine and uracil residues on single stranded RNA, hydrolysing the phosphodiester bond between the 5 prime ribose of one nucleotide and the 3 prime phosphate of the neighbouring pyrimidine so that the 2 prime, 3 prime cyclic phosphate resolves to the corresponding 3 prime nucleotide phosphate. Activity is highest on single stranded RNA and depends on salt: at low salt concentrations from 0 to 100 mM sodium chloride the enzyme cuts single stranded RNA, double stranded RNA and the RNA strand of RNA-DNA hybrids, while at 0.3 M and above it cuts only single stranded RNA. Supplied at 10 mg/mL in 50 mM Tris-HCl pH 7.4 with 50% glycerol and free of DNase and protease, it is most often used to clear RNA from plasmid DNA or genomic DNA preparations and in RNase protection assays and RNA sequence analysis. |
| Intended Use | RNA removal during plasmid and genomic DNA preparation, RNase protection assays and RNA sequence analysis |
| Principle / Technology | Endoribonucleolytic cleavage after cytosine and uracil residues via a 2 prime, 3 prime cyclic phosphate intermediate |
| Sample Type | RNA contamination in DNA preparations; RNA substrates for analysis |
| Specificity | Degrades C and U residues of single stranded RNA; at high salt cuts single stranded RNA only |
| Reaction Conditions / Protocol | Treat the sample with the enzyme at the working concentration, then remove the enzyme before downstream use |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 1 mL |
| Key Features | Kunitz unit based activity; salt dependent single strand specificity |
| Purity | Free of DNase and protease |
| Concentration | 10 mg/mL |
| Shipping Conditions | Ice pack shipping |
| Recommended Buffer System | 50 mM Tris-HCl pH 7.4 with 50% glycerol |
| Readouts / Downstream Assays | RNA clearance verified by gel electrophoresis |
For in vitro diagnostic use.
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