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| Product Name | Ribonuclease H (5 U/uL) |
| Catalog No. | IVDRM-0330 |
| Description | An endoribonuclease that hydrolyses phosphodiester bonds of the RNA strand in RNA-DNA hybrids while leaving single-stranded and double-stranded DNA or RNA untouched. This hybrid specificity underpins its roles in second-strand cDNA synthesis after first-strand reactions, RNA removal in RT-PCR and RT-qPCR for cleaner detection, poly(A) trimming following Oligo(dT) hybridisation and site-specific RNA cleavage, and it feeds RNase H-based rRNA depletion workflows in sequencing library construction. |
| Intended Use | Removal of mRNA before second-strand cDNA synthesis; RNA digestion after first-strand RT-PCR and RT-qPCR; poly(A) removal after Oligo(dT) hybridisation; site-specific RNA cleavage; rRNA depletion workflows. |
| Principle / Technology | Endonucleolytic cleavage restricted to the RNA strand of RNA-DNA hybrids. |
| Sample Type | RNA-DNA hybrid substrates |
| Performance Range / Specifications | Specific hybrid-targeted hydrolysis without digestion of single-stranded or double-stranded DNA or RNA. |
| Specificity | Strictly hybrid-dependent activity. |
| Reaction Conditions / Protocol | Incubate the enzyme with hybrid substrates under buffer conditions, then purify the products. |
| Components / Formulation | Ribonuclease H in storage buffer. |
| Storage Conditions | -25 to -15 °C |
| Shelf Life | 2 years |
| Package Specifications | 100 U, 500 U, 5000 U, 20 mL |
| Product Form | Enzyme solution |
| Key Features | Hybrid-specific cleavage; broad cDNA and depletion utility; bulk format available. |
| Concentration | 5 U/uL |
| Readouts / Downstream Assays | RNA-depleted cDNA or cleaved RNA products |
For in vitro diagnostic use.
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