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| Product Name | Reduced Serum Medium (for Transfection, with Phenol Red) |
| Catalog No. | RESK-0635 |
| Description | Reduced Serum Medium (for Transfection, with Phenol Red) is a modified minimum essential medium supplemented with insulin, transferrin, thymosin, hypoxanthine and multiple trace elements. These components compensate for the reduced serum concentration, allowing the amount of fetal bovine serum added to be reduced by 50% without affecting cell growth rate and while maintaining normal cell morphology. This product is suitable for the complexation of nucleic acids by cationic lipid transfection reagents and for transient transfection of cells. It can be used for various adherent and suspension mammalian cells, including CHO, BHK-21, HEK, Sp2 and primary cells. |
| Intended Use | Cell culture and transfection medium for the complexation of nucleic acids with cationic lipid transfection reagents and for transient cell transfection; supports cell culture with fetal bovine serum supplementation reduced by 50% while maintaining cell growth and morphology. |
| Principle / Technology | A modified minimum essential medium supplemented with insulin, transferrin, thymosin, hypoxanthine and multiple trace elements that compensate for reduced serum concentration, enabling a 50% reduction in fetal bovine serum usage without compromising cell growth or morphology. |
| Sample Type | Cells: adherent and suspension mammalian cells, including CHO, BHK-21, HEK, Sp2 and primary cells; applicable species: human, mouse, hamster. |
| Performance Range / Specifications | Sterility: sterile filtered Endotoxin: <= 0.5 EU/mL Osmolality: 280-320 mOsm/kg pH: 7.0-7.4 Buffer system: sodium bicarbonate buffer system (2.4 g/L) Contains Phenol Red |
| Reaction Conditions / Protocol | This product uses a sodium bicarbonate buffer system (2.4 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH. |
| Components / Formulation | Modified minimum essential medium supplemented with insulin, transferrin, thymosin, hypoxanthine and multiple trace elements; contains Phenol Red; sodium bicarbonate buffer system (2.4 g/L). |
| Storage Conditions | Store at 2-8 °C, protected from light. |
| Shelf Life | 1 year. |
| Package Specifications | 500 mL. |
| Product Form | Liquid. |
| Quality Control | Sterile filtered; endotoxin <= 0.5 EU/mL; osmolality 280-320 mOsm/kg; pH 7.0-7.4. |
| pH Range / Optimal pH | 7.0-7.4. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Suitable for the complexation of nucleic acids with cationic lipid transfection reagents and for transient transfection of various adherent and suspension mammalian cells, including CHO, BHK-21, HEK, Sp2 and primary cells. |
| Recommended Buffer System | Sodium bicarbonate buffer system (2.4 g/L). |
| Application Notes / Precautions | 1. A 5-10% CO2 environment is required to maintain physiological pH. 2. Store at 2-8 °C protected from light. |
For research use only, not for clinical use.
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