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| Product Name | Recombinant Trypsin-EDTA Solution (1x), Phenol Red-Free, Animal Origin-Free |
| Catalog No. | RESK-0247 |
| Description | This recombinant trypsin digestion solution is formulated by sterile filtration using a high-purity, high-specific-activity recombinant protease expressed by a genetically engineered strain as the main raw material. The product is sterile, contains 1 mM EDTA, has a pH of 7.0-7.6, and is free of animal-derived components with a chemically defined composition, effectively avoiding exogenous viral contamination and ensuring high biosafety. It is suitable for the dissociation of mesenchymal stem cells and other weakly adherent cells during passaging under serum-free conditions. The enzyme acts gently, causes minimal cell damage, and delivers high cell viability. |
| Intended Use | Gentle dissociation of mesenchymal stem cells (MSCs) and other weakly adherent cells during passaging under serum-free conditions. For research use only. |
| Principle / Technology | Recombinant trypsin produced by a genetically engineered strain cleaves peptide bonds at the C-terminal side of lysine and arginine residues to detach adherent cells; EDTA (1 mM) chelates calcium and magnesium ions to enhance dissociation. The solution is prepared by sterile filtration and is free of animal-derived components. |
| Sample Type | Mesenchymal stem cells and other weakly adherent cells cultured under serum-free conditions. |
| Performance Range / Specifications | Appearance: colorless, clear liquid pH: 7.0-7.6 Osmolality: 270-340 mOsmol/kg Bacterial endotoxin: <1 EU/mL Mycoplasma: negative Sterility: sterile EDTA: 1 mM |
| Specificity | High-purity, high-specific-activity recombinant trypsin; gentle action with minimal cell damage. Continuous digestion for 30 min does not affect the viability or subsequent passaging of mesenchymal stem cells. |
| Reaction Conditions / Protocol | Example: passaging umbilical cord-derived mesenchymal stem cells in a T75 flask. 1. Take out the recombinant trypsin digestion solution and allow it to equilibrate to room temperature. 2. Culture the cells until they reach 80%-90% confluence, then proceed with passaging or harvest. 3. Washing: aspirate the medium from the T75 flask, add 10-15 mL of calcium- and magnesium-free PBS to rinse the cells, and discard the PBS. 4. Digestion: add 3 mL of the recombinant trypsin digestion solution to cover the entire cell growth surface, and digest at 37 °C (or room temperature) for 3-5 min. Examine under a microscope; cells should retract, round up, and detach. Add two volumes of culture medium to stop the digestion, pipette to disperse the cells into a single-cell suspension, and perform cell counting. Note: if most cells are rounded but not detached, gently tap the flask wall to dislodge them; extend the digestion time if needed. 5. Collection: centrifuge at 300 g for 5 min and discard the supernatant. 6. Proceed with downstream operations as required. |
| Components / Formulation | Sterile-filtered 1x recombinant trypsin solution containing 1 mM EDTA; phenol red-free; animal origin-free; chemically defined; serum-free. |
| Storage Conditions | Store at 2-8 °C protected from light. |
| Shelf Life | 1 year when stored at 2-8 °C protected from light. |
| Package Specifications | 100 mL 5 x 100 mL |
| Product Form | Liquid, 1x ready-to-use solution |
| Quality Control | Appearance: colorless, clear liquid pH: 7.0-7.6 Osmolality: 270-340 mOsmol/kg Bacterial endotoxin: <1 EU/mL Mycoplasma: negative Sterility: sterile Manufactured under cGMP requirements. |
| Purity | High purity, high specific activity recombinant trypsin (exact value not specified). |
| Concentration | 1x working concentration, ready to use (exact enzyme concentration not specified). |
| Activity / Unit Definition | High specific activity (exact value not specified). |
| Source / Origin | Recombinant trypsin expressed by a genetically engineered strain; animal origin-free (AOF), serum-free, free of exogenous viral contamination risk. |
| pH Range / Optimal pH | pH 7.0-7.6 |
| Expiration Date / Stability | Valid for 1 year when stored at 2-8 °C protected from light. |
| Regulatory / Compliance | Recombinant trypsin manufactured according to cGMP requirements; serum-free and animal origin-free. For research use only. |
| Compatibility | Phenol red-free formulation suitable for flow cytometry (no phenol red interference with fluorescence signals), hormone-related research (e.g., estrogen-sensitive studies), and serum-free or specialized culture systems where phenol red may interfere with sodium-potassium balance or medium composition. |
| Recommended Buffer System | Contains 1 mM EDTA; rinse cells with calcium- and magnesium-free PBS before digestion; stop digestion by adding two volumes of culture medium. |
| Application Notes / Precautions | 1. Gentle digestion: minimal cell damage; continuous digestion for 30 min does not affect MSC viability or subsequent passaging. 2. Efficient and convenient: simply dilute with culture medium or culture supernatant to stop the reaction; no inhibitor is required. 3. Time-saving: no thawing required, short digestion time for rapid MSC detachment. 4. Safe and reliable: recombinant trypsin produced under cGMP requirements; serum-free and free of animal-derived components. |
| Recognition Site | Cleaves peptide bonds at the C-terminal side of lysine and arginine residues. |
| Heat Inactivation | Not applicable; digestion is stopped by dilution with culture medium or culture supernatant, without the need for an inhibitor. |
For research use only, not for clinical use.
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