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| Product Name | Recombinant RNase A, Yeast Expressed, DNase-Free (10 mg/mL) |
| Catalog No. | IVDRM-0552 |
| Description | Ribonuclease A engineered by protein technology from the bovine pancreas gene and obtained through yeast expression and purification, this recombinant RNase A carries none of the bacterial endotoxin associated with prokaryotic expression systems. Functionally it is a non-specific endoribonuclease that hydrolyses the phosphodiester bond between the 5 prime ribose of a nucleotide and the 3 prime phosphate of the adjacent pyrimidine, so the resulting 2 prime, 3 prime cyclic phosphate resolves to the corresponding 3 prime nucleotide phosphate, and it degrades single stranded RNA specifically at cytosine and uracil residues with highest effect on single stranded substrates. The enzyme is very stable, and the preparation is free of endonuclease and exonuclease contamination and free of protease residue, which suits removal of RNA contamination from DNA or protein samples. |
| Intended Use | Removal of RNA contamination from DNA or protein samples |
| Principle / Technology | Endoribonucleolytic cleavage after cytosine and uracil residues via a 2 prime, 3 prime cyclic phosphate intermediate |
| Sample Type | RNA contamination in DNA or protein preparations |
| Specificity | Degrades single stranded RNA specifically at C and U residues |
| Reaction Conditions / Protocol | Treat the sample with the enzyme and remove it before downstream use |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 1 year |
| Package Specifications | 1 mL; 5 mL; 10 mL |
| Key Features | Yeast expression avoids bacterial endotoxin; highly stable enzyme |
| Purity | Free of endonuclease and exonuclease contamination; free of protease residue; no bacterial endotoxin |
| Concentration | 10 mg/mL |
| Source / Origin | Bovine pancreas gene, yeast expressed |
| Readouts / Downstream Assays | RNA clearance verified by gel electrophoresis |
For in vitro diagnostic use.
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