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| Product Name | Recombinant Protein A/G with C-Terminal Cysteine |
| Catalog No. | IGM-HMM-0078 |
| Description | A recombinant fusion protein containing the IgG-binding domains of Protein A and Protein G. Cell-wall, membrane-binding and albumin-binding regions are removed to reduce nonspecific interactions, while a terminal cysteine supports oriented immobilization. |
| Intended Use | Affinity-ligand coupling to agarose for antibody purification and coupling to magnetic beads for immunoprecipitation or co-immunoprecipitation studies. |
| Principle / Technology | The combined Protein A and Protein G domains bind the Fc region of a broad range of mammalian IgG molecules. The terminal cysteine facilitates covalent immobilization on activated solid supports. |
| Specificity | Broad binding to IgG from multiple mammalian species without an albumin-specific binding site. |
| Components / Formulation | Recombinant Protein A/G fusion protein with a C-terminal cysteine. |
| Storage Conditions | Store at -30 to -15°C. Long-term storage at 2-8°C is not recommended; sterile filtration is advised when refrigerated storage is required. |
| Shelf Life | 3 years when stored as directed. Avoid repeated freeze-thaw cycles. |
| Package Specifications | 1 mg; 10 mg; 100 mg; 500 mg. Custom package sizes are available. |
| Product Form | Recombinant affinity protein. |
| Key Features | Broad IgG species coverage; high binding capacity; strong specificity; reduced nonspecific binding; immobilization-ready terminal cysteine. |
| Source / Origin | Recombinantly expressed in Escherichia coli. |
| Shipping Conditions | Ship at 0°C or below. |
| Compatibility | Agarose affinity media and magnetic-bead coupling platforms. |
| Application Notes / Precautions | Avoid repeated freeze-thaw cycles. Use an appropriate cysteine-reactive coupling chemistry when immobilizing the ligand. |
For research use only, not for clinical use.
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