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| Product Name | Recombinant Human Insulin |
| Catalog No. | RESK-0480 |
| Description | Insulin is a double-chain (alpha, beta) polypeptide hormone secreted by the beta cells of the pancreatic islets. It consists of 51 amino acids, with 21 amino acids in the alpha chain and 30 amino acids in the beta chain. The two chains are connected by two disulfide bonds, and the alpha chain itself contains an additional intrachain disulfide bond. During insulin secretion, preproinsulin carries a 23-30 amino acid signal peptide at its amino terminus, which directs preproinsulin to the endoplasmic reticulum, where it is immediately cleaved, releasing proinsulin. Proinsulin consists of the insulin beta chain, C-peptide, and alpha chain; it is then transferred to the Golgi apparatus, where it is packaged and converted into insulin, which is released into the blood together with the C-peptide. Insulin is the only known protein hormone in the body that lowers blood glucose; it not only regulates cellular uptake, utilization, and storage of glucose, amino acids, and fatty acids, but also inhibits the degradation of glycogen, protein, and fat. This product is full-length human insulin expressed in Escherichia coli, with a molecular weight of approximately 5800 Da. Its structure is identical to that of naturally derived human insulin. It is commonly used as a cell culture supplement at a typical working concentration of 1-10 ug/mL. |
| Intended Use | Used as a supplement in cell culture, typically at a working concentration of 1-10 ug/mL. For research use only; not for live animal experiments. |
| Principle / Technology | Full-length human insulin expressed in Escherichia coli; its structure is identical to that of naturally derived human insulin. Insulin regulates cellular uptake, utilization, and storage of glucose, amino acids, and fatty acids, and inhibits the degradation of glycogen, protein, and fat. |
| Performance Range / Specifications | Molecular weight: approximately 5800 Da High purity, high activity, low endotoxin, high batch-to-batch consistency |
| Reaction Conditions / Protocol | Recombinant human insulin is supplied as a powder. It is generally dissolved in dilute hydrochloric acid solution (10 mM) to prepare a stock solution containing 1-2 mg per mL, then sterilized by filtration. The filtrate can be aliquoted and stored at -20 °C for 6 months. Repeated freeze-thaw cycles should be avoided. |
| Storage Conditions | Lyophilized powder: store at -25 to -15 °C. Stock solution: store at -25 to -15 °C. |
| Shelf Life | Lyophilized powder: 3 years. Stock solution: 6 months. |
| Package Specifications | 25 mg, 100 mg, 500 mg, 1 g |
| Product Form | Powder |
| Quality Control | High purity, high activity, low endotoxin, high batch-to-batch consistency. |
| Concentration | Typical working concentration in cell culture: 1-10 ug/mL; stock solution: 1-2 mg/mL in 10 mM dilute hydrochloric acid. |
| Molecular Weight | Approximately 5800 Da |
| Source / Origin | Full-length human insulin expressed in Escherichia coli (recombinant); structure identical to naturally derived human insulin. |
| Regulatory / Compliance | For research use only. |
| Recommended Buffer System | Dilute hydrochloric acid (10 mM) or dilute acetic acid. |
| Application Notes / Precautions | This product is not for live animal experiments; insulin tolerance can be observed in human cells. No matching buffer is provided; prepare the stock solution with dilute acetic acid or dilute hydrochloric acid according to the instructions. Avoid repeated freeze-thaw cycles of the stock solution. Literature reports describe the use of insulin for adipocyte induction, but this application has not been validated with this product. |
| Batch-to-Batch Consistency | High batch-to-batch consistency. |
For research use only, not for clinical use.
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