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| Product Name | Recombinant DNase I, Yeast Expressed, RNase-Free |
| Catalog No. | IVDRM-0482 |
| Description | Deoxyribonuclease I is an endonuclease that digests single or double stranded DNA by hydrolysing phosphodiester bonds to release monodeoxyribonucleotides and oligodeoxyribonucleotides carrying 5 prime phosphate and 3 prime hydroxyl groups. Its working pH range is 7 to 8, activity depends on calcium ions and can be stimulated by divalent metals such as cobalt, manganese and zinc, and the pattern of cutting shifts with the metal present, from random nicking of duplex DNA in the presence of magnesium to same site double strand cutting that leaves blunt or one to two nucleotide protruding ends when manganese is used. Produced in a yeast expression system and free of RNase contamination, it removes DNA from RNA samples and improves RNA purity during extraction. |
| Intended Use | Removal of DNA from RNA samples and improvement of RNA purity during extraction |
| Principle / Technology | Endonucleolytic hydrolysis of DNA phosphodiester bonds, calcium dependent and stimulated by divalent metal ions |
| Sample Type | RNA samples; single and double stranded DNA |
| Reaction Conditions / Protocol | Digest the RNA sample with DNase I under magnesium or manganese conditions according to the required cutting pattern, then remove the enzyme |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 250 T; 50 T |
| Key Features | Yeast expression system; RNase free; efficient DNA digestion for RNA workflows |
| Purity | RNase free |
| Source / Origin | Yeast expression system |
| Readouts / Downstream Assays | Agarose gel electrophoresis for RNase residue and RNA integrity |
For in vitro diagnostic use.
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