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| Product Name | Recombinant Collagenase Type II (5 U/μL) |
| Catalog No. | RESK-0230 |
| Description | Recombinant Collagenase Type II (COL H) is a type II collagenase recombinantly expressed in Escherichia coli (E. coli), with its gene derived from Clostridium histolyticum. This collagenase specifically recognizes and efficiently cleaves native triple-helical collagen and the non-triple-helical Pro-X-Gly-Pro sequence (cleavage occurs at the peptide bond between the neutral amino acid X and glycine Gly). It is generally used for the isolation of cells from liver, bone, thyroid, heart, and salivary gland tissues. |
| Intended Use | Generally used for the isolation of cells from liver, bone, thyroid, heart, and salivary gland tissues. |
| Principle / Technology | Recombinantly expressed type II collagenase that specifically recognizes and efficiently cleaves native triple-helical collagen and the non-triple-helical Pro-X-Gly-Pro sequence at the peptide bond between the neutral amino acid X and glycine Gly. |
| Sample Type | Liver, bone, thyroid, heart, and salivary gland tissues. |
| Specificity | Specifically recognizes and efficiently cleaves native triple-helical collagen and the non-triple-helical Pro-X-Gly-Pro sequence (cleavage between the neutral amino acid X and glycine Gly). |
| Storage Conditions | Store at -25 to -15 °C. |
| Shelf Life | 2 years when stored at -25 to -15 °C. |
| Package Specifications | 500 U 2500 U |
| Product Form | Liquid solution, 5 U/μL. |
| Concentration | 5 U/μL. |
| Source / Origin | Recombinantly expressed in Escherichia coli (E. coli); gene derived from Clostridium histolyticum. Recombinant source; free of animal-derived components (animal-origin-free). |
| Expiration Date / Stability | Stable for 2 years at -25 to -15 °C. |
| Regulatory / Compliance | Recombinant source; free of animal-derived components. |
| Recognition Site | Pro-X-Gly-Pro sequence; cleaves the peptide bond between the neutral amino acid X and glycine (Gly); also cleaves native triple-helical collagen. |
For research use only, not for clinical use.
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