- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Recombinant Cell Dissociation Enzyme (1x), with Phenol Red |
| Catalog No. | RESK-0245 |
| Description | This product is a non-animal-origin recombinant trypsin that cleaves peptide bonds at the C-terminal side of lysine and arginine residues and can directly replace conventional trypsin in existing protocols. It is suitable for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, and A529 cells. The product is of high purity, exhibits stronger specificity than natural trypsin preparations, and causes low damage to cells. It is dissolved in DPBS with 1 mM EDTA, contains phenol red, is stable at room temperature, and is convenient to use. Its gentle dissociation helps preserve cell viability and cell surface protein integrity, making it well suited for primary cells and other applications where surface marker integrity must be maintained, and it can be used directly in serum-free systems. |
| Intended Use | Gentle dissociation of adherent mammalian cells (e.g., CHO, HEK 293, A529), including primary cells and other applications requiring preservation of cell surface protein integrity; a direct, animal-free replacement for trypsin in existing protocols; suitable for serum-free culture systems. |
| Principle / Technology | Recombinant trypsin of non-animal origin that cleaves peptide bonds at the C-terminal side of lysine and arginine residues, degrading adhesion proteins to gently release adherent cells. High purity provides greater specificity than natural trypsin preparations and reduces cell damage; the enzyme is room-temperature stable for convenient handling. |
| Sample Type | Adherent mammalian cells, including CHO, HEK 293, and A529 cells, and primary cells |
| Specificity | Cleaves peptide bonds at the C-terminal side of lysine and arginine residues; stronger specificity than natural trypsin preparations. |
| Reaction Conditions / Protocol | Designed as a direct replacement for trypsin in existing protocols. 1. Before use, prewarm the enzyme and complete growth medium to 37 °C; minimize residence time. Note: the enzyme can also be used at room temperature for many cell types. 2. Aspirate the spent medium and discard. 3. Rinse the cell monolayer with 5 mL of calcium- and magnesium-free DPBS; aspirate and discard. 4. Add an appropriate volume of enzyme to the culture flask (e.g., 5 mL for a 75 cm2 flask), ensuring the monolayer is completely covered. 5. Incubate at 37 °C until the cells detach. Monitor the monolayer with an inverted microscope to confirm complete detachment from the flask surface; gently tap the flask if necessary. 6. Add 5-10 mL of prewarmed complete medium to the flask and tilt the flask in all directions to rinse thoroughly. Transfer the cell suspension to a 15 mL centrifuge tube. 7. Centrifuge at 100 g for 5-10 min. 8. Discard the supernatant and resuspend the cells in 2-5 mL of prewarmed complete medium. |
| Components / Formulation | Recombinant trypsin (non-animal origin) dissolved in DPBS with 1 mM EDTA With phenol red Supplied as a 1x ready-to-use solution |
| Storage Conditions | Store at room temperature, protected from light. |
| Shelf Life | 2 years |
| Package Specifications | 100 mL |
| Product Form | Liquid, 1x ready-to-use solution |
| Quality Control | High purity; stronger specificity than natural trypsin preparations. |
| Purity | High purity |
| Source / Origin | Recombinant, non-animal origin |
| Shipping Conditions | Room temperature |
| Expiration Date / Stability | Stable for 2 years at room temperature when protected from light; room-temperature stable for convenient use. |
| Compatibility | Direct replacement for trypsin in existing cell dissociation protocols; can be used directly in serum-free culture systems; compatible with many cell types at room temperature. |
| Recommended Buffer System | DPBS (calcium- and magnesium-free) with 1 mM EDTA |
| Application Notes / Precautions | Prewarm the enzyme and complete growth medium to 37 °C before use and minimize residence time. Rinse the monolayer with calcium- and magnesium-free DPBS before adding the enzyme. Ensure the monolayer is fully covered, and confirm complete detachment with an inverted microscope. |
| Recognition Site | Cleaves peptide bonds at the C-terminal side of lysine and arginine residues. |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
