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| Product Name | Recombinant Cell Dissociation Enzyme (1x), Phenol Red-Free |
| Catalog No. | RESK-0246 |
| Description | This product is a ready-to-use, animal origin-free recombinant enzyme that specifically cleaves peptide bonds at the C-terminal side of lysine and arginine residues. It serves as a direct replacement for conventional trypsin in the dissociation of a wide range of adherent mammalian cells, including CHO, HEK 293, and A529 cells. With high purity and greater specificity than natural trypsin preparations, it causes minimal damage to cells. The enzyme is supplied in DPBS containing 1 mM EDTA, is stable at room temperature, and is convenient to use. The phenol red-free formulation is especially suitable for flow cytometry (avoiding phenol red interference with fluorescence signals), hormone-related research (e.g., estrogen-sensitive studies with breast cancer or endometrial cells), and serum-free or specialized culture systems. |
| Intended Use | Gentle dissociation of adherent mammalian cells during routine passaging, particularly primary cells and applications requiring intact cell-surface proteins. For research use only. |
| Principle / Technology | Recombinant enzyme of non-animal origin that hydrolyzes peptide bonds at the C-terminal side of lysine and arginine residues, releasing adherent cells from the culture surface; designed as a direct, drop-in replacement for trypsin in existing protocols. |
| Sample Type | Adherent mammalian cells (e.g., CHO, HEK 293, A529), including primary cells requiring preservation of cell-surface protein integrity. |
| Performance Range / Specifications | Appearance: clear liquid Formulation: DPBS with 1 mM EDTA, phenol red-free Working concentration: 1x, ready to use Stability: stable at room temperature; also stable at 2-8 °C and -20 to -5 °C (protected from light) for up to 24 months |
| Specificity | Cleaves peptide bonds at the C-terminal side of lysine and arginine residues. Higher specificity than natural trypsin preparations, with low cytotoxicity and minimal impact on cell viability and surface proteins. |
| Reaction Conditions / Protocol | 1. Prewarm the enzyme solution and complete growth medium to 37 °C; minimize standing time. The enzyme may also be used at room temperature for many cell types. 2. Aspirate and discard the spent medium. 3. Rinse the cell monolayer with 5 mL of calcium- and magnesium-free DPBS; aspirate and discard. 4. Add an appropriate volume of the enzyme solution to the flask (e.g., 5 mL for a 75 cm2 flask), ensuring the monolayer is fully covered. 5. Incubate at 37 °C until the cells detach. Monitor the monolayer with an inverted microscope; tap the flask gently if necessary to dislodge cells. 6. Add 5-10 mL of prewarmed complete medium to the flask and tilt in all directions to rinse thoroughly. Transfer the cell suspension to a 15 mL centrifuge tube. 7. Centrifuge at 100 g for 5-10 min. 8. Discard the supernatant and resuspend the cells in 2-5 mL of prewarmed complete medium. |
| Components / Formulation | Recombinant cell dissociation enzyme (animal origin-free) dissolved in DPBS containing 1 mM EDTA; phenol red-free; 1x ready-to-use solution. |
| Storage Conditions | Store at room temperature protected from light. The product is also stable at 2-8 °C or at -20 to -5 °C (protected from light) for up to 24 months. |
| Shelf Life | 2 years (24 months) when stored as recommended. |
| Package Specifications | 100 mL |
| Product Form | Liquid, 1x ready-to-use solution |
| Quality Control | High purity; free of animal-derived components; greater specificity and lower cell damage than natural trypsin preparations. |
| Purity | High purity (exact value not disclosed by the manufacturer). |
| Concentration | The enzyme concentration in this 1x solution is proprietary. |
| Source / Origin | Recombinant enzyme of non-animal origin (animal origin-free). |
| Expiration Date / Stability | Stable for 24 months at room temperature, 2-8 °C, or -20 to -5 °C when protected from light. |
| Regulatory / Compliance | For research use only. Not for diagnostic or therapeutic use. |
| Compatibility | Phenol red-free formulation compatible with flow cytometry, hormone-related research (estrogen-sensitive systems), and serum-free or specialized culture systems where phenol red may interfere with fluorescence signals, sodium-potassium balance, or medium composition. |
| Recommended Buffer System | Supplied in DPBS with 1 mM EDTA; rinse cells with calcium- and magnesium-free DPBS before dissociation. |
| Application Notes / Precautions | 1) Dilution alone inactivates the enzyme; no trypsin inhibitor (e.g., FBS) is required. 2) The product is stable at 2-8 °C and -20 to -5 °C for up to 24 months when protected from light. 3) For your safety, wear a lab coat and disposable gloves during handling. 4) This product is for research use only. |
| Recognition Site | Cleaves peptide bonds at the C-terminal side of lysine and arginine residues. |
| Heat Inactivation | Not applicable; the enzyme is inactivated by simple dilution and does not require a trypsin inhibitor such as FBS. |
For research use only, not for clinical use.
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