- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Recombinant Broad-Spectrum Endonuclease for Nucleic Acid Removal (250-300 U/uL) |
| Catalog No. | IVDRM-0368 |
| Description | This non-specific endonuclease, originally derived from Serratia marcescens and engineered for expression in E. coli, cleaves at any inter-nucleotide position and digests nucleic acids completely into 2 to 5 base 5-monophosphate oligonucleotides. It degrades all forms of DNA and RNA, including double stranded, single stranded, linear, circular, native and denatured, even under harsh conditions such as 6 M urea, 0.1 M guanidine hydrochloride, 0.4% Triton X-100, 0.1% SDS, 1 mM EDTA or 1 mM PMSF. Applications include reducing viscosity of cell supernatants and lysates to improve protein purification efficiency, removing nucleic acid from biopharmaceutical products, and preventing clumping of human peripheral blood mononuclear cells in cell therapy and vaccine research. |
| Intended Use | Nucleic acid removal from biopharmaceutical products; viscosity reduction in protein purification; PBMC clumping prevention in cell therapy and vaccine research |
| Principle / Technology | Non-specific endonucleolytic cleavage digesting DNA and RNA into short 5-monophosphate oligonucleotides |
| Sample Type | Cell supernatants; cell lysates; peripheral blood mononuclear cells |
| Storage Conditions | -15 to -25°C |
| Shelf Life | 2 years |
| Package Specifications | 25 KU; 50 KU; 100 KU |
| Product Form | Sterile liquid enzyme |
| Key Features | Broad condition tolerance; digests all DNA and RNA forms; drug master file filed; safety tested for sterility, endotoxin, mycoplasma and adventitious viruses |
| Purity | >=99% (HPLC) |
| Concentration | 250-300 U/uL |
| Activity / Unit Definition | Specific activity >=1.5E6 U/mg; optimal pH 8.0 with working range pH 6 to 10; optimal temperature 37°C with working range 0 to 42°C; cofactor 1-10 mM Mg2+ |
| Source / Origin | Serratia marcescens derived, expressed in E. coli |
| Shipping Conditions | Dry ice |
| Recommended Buffer System | 20 mM Tris-HCl pH 8.0, 2 mM MgCl2, 20 mM NaCl, 50% glycerol |
For in vitro diagnostic use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
