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| Product Name | Recombinant Bovine Holo-Transferrin (CHO-Expressed, Lyophilized) |
| Catalog No. | RERE-0026 |
| Description | Bovine transferrin is the classic bioavailable iron carrier for cell and tissue culture, supplying the iron that fast-growing cells need while restraining free-iron chemistry that can damage them. This recombinant holo (iron-saturated) version is produced in CHO cells covering the Asp20-Pro704 mature sequence (accession Q29443) with no fusion tag, purified to at least 95% by SDS-PAGE under reducing and non-reducing conditions, and released at endotoxin of 10 EU/mg or lower by the LAL method. Activity is benchmarked in a serum-free MDCK proliferation assay with an ED50 of 0.5-2 μg/mL, and the optimum for a given cell type should be titrated against the application. The protein is lyophilized from a 0.22 μm-filtered sterile water solution and travels with high batch-to-batch consistency, making it a dependable serum-replacement component for defined and serum-free media. |
| Intended Use | Iron delivery for serum-free and defined cell culture; support of cell and tissue proliferation; supplement for media replacing animal serum components. |
| Principle / Technology | The iron-loaded protein binds its cell-surface receptor and delivers iron through receptor-mediated endocytosis, giving cells a controlled, bioavailable iron supply. |
| Sample Type | Lyophilized powder reconstituted with sterile deionised water. |
| Performance Range / Specifications | Recombinant bovine holo-transferrin from CHO cells; at least 95% purity by SDS-PAGE; endotoxin of 10 EU/mg or lower by LAL; ED50 of 0.5-2 μg/mL in a serum-free MDCK proliferation assay; high lot-to-lot consistency. |
| Sensitivity / LOD | Does not apply; this product induces a model rather than generating a quantifiable signal. |
| Specificity | Molecular weight 75.8 kDa; accession Q29443; sequence Asp20-Pro704; expression system CHO; tag none; formulation lyophilized from a 0.22 μm-filtered sterile water solution; purity at least 95% by SDS-PAGE. |
| Reaction Conditions / Protocol | Reconstitute the lyophilized protein with sterile deionised water, aliquot on first use to avoid repeated freeze-thaw, and add to culture medium at the concentration suited to the cell type. |
| Components / Formulation | One vial of lyophilized recombinant bovine holo-transferrin (100 mg or 1 g). |
| Storage Conditions | Store lyophilized material at -25 °C to -15 °C; shelf life 1 year from receipt. After reconstitution, 2-8 °C holds for 7-10 days and -85 °C to -65 °C holds for 6 months. |
| Shelf Life | 1 year lyophilized; 7-10 days at 2-8 °C or 6 months at -85 °C to -65 °C after reconstitution. |
| Package Specifications | 100 mg or 1 g per vial |
| Product Form | Lyophilized powder. |
| Quality Control | Purity of at least 95% by SDS-PAGE under reducing and non-reducing conditions; endotoxin of 10 EU/mg or lower by LAL; activity confirmed by MDCK proliferation assay with ED50 of 0.5-2 μg/mL. |
| Key Features | - CHO-expressed holo-transferrin, accession Q29443, no tag - ED50 of 0.5-2 μg/mL in a serum-free MDCK proliferation assay - Endotoxin of 10 EU/mg or lower; at least 95% purity by SDS-PAGE - High lot-to-lot consistency for defined media |
| Purity | ≥95% by SDS-PAGE under reducing and non-reducing conditions, visualised by Coomassie staining. |
| Concentration | Supplied as a solid; reconstitution concentration is set by the user. |
| Activity / Unit Definition | ED50 of 0.5-2 μg/mL in a serum-free cell proliferation assay using MDCK canine kidney epithelial cells; the optimal concentration depends on cell type and application. |
| Molecular Weight | 75.8 kDa |
| Source / Origin | Recombinant bovine transferrin expressed in CHO cells, covering the Asp20-Pro704 mature sequence (accession Q29443), iron-saturated (holo) form, no fusion tag. |
| pH Range / Optimal pH | Does not apply; pH is not a meaningful specification for this product type. |
| Shipping Conditions | Shipped chilled. |
| Expiration Date / Stability | Lyophilized material is stable through the stated shelf life frozen; reconstituted material holds 7-10 days at 2-8 °C or 6 months at -85 °C to -65 °C. |
| Regulatory / Compliance | Restricted to research applications; not for clinical use. |
| Compatibility | Suitable for serum-free and defined media across cell lines and primary cultures; titrate the dose to the cell type. |
| Recommended Buffer System | Lyophilized from a 0.22 μm-filtered sterile water solution; reconstitute with sterile deionised water. |
| Application Notes / Precautions | Aliquot on first reconstitution to avoid repeated freeze-thaw; keep reconstituted protein chilled and use within the stated holding windows. |
| Batch-to-Batch Consistency | High lot-to-lot consistency is maintained through controlled expression and release testing on purity, endotoxin and activity. |
| Model Type | Recombinant cell culture protein supplement |
| Starting Material / Tissue Site | CHO-expressed recombinant bovine holo-transferrin |
| Delivery Standard | 100 mg or 1 g lyophilized vial |
| Readouts / Downstream Assays | Serum-free proliferation assays (reference ED50 of 0.5-2 μg/mL on MDCK cells). |
For research use only, not for clinical use.
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