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| Product Name | RecJ Exonuclease (30 U/uL) |
| Catalog No. | IVDRM-0568 |
| Description | From the Escherichia coli K12 strain, RecJ Exonuclease is a magnesium-dependent exonuclease that specifically recognises and acts on the 5 prime ends of single stranded DNA, equivalent in enzymatic function to commercially available RecJf Exonuclease. It degrades linear single stranded DNA in the 5 prime to 3 prime direction, trims double stranded DNA 5 prime overhangs longer than six nucleotides rather than simply producing flush ends, acts with similar efficiency on single stranded DNA carrying either a 5 prime phosphate or a 5 prime hydroxyl, and shows no activity on RNA. The enzyme is at least 95% pure, has low nickase and RNase residue and is fully inactivated by heating at 65°C for 20 minutes, which suits purity-critical downstream applications. |
| Intended Use | Degradation of single stranded DNA and trimming of 5 prime overhangs on double stranded DNA |
| Principle / Technology | Magnesium dependent 5 prime to 3 prime exonucleolytic digestion specific for single stranded DNA |
| Sample Type | Single stranded DNA and double stranded DNA with 5 prime overhangs |
| Specificity | Acts on 5 prime phosphate and 5 prime hydroxyl ssDNA alike; overhangs longer than six nucleotides on dsDNA; no RNA activity |
| Reaction Conditions / Protocol | Digest with the enzyme in a magnesium-containing buffer and stop by heating at 65°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 1500 U; 6000 U |
| Key Features | Heat inactivatable at 65°C; suited to purity-critical downstream applications |
| Purity | Greater than or equal to 95%; low nickase and RNase residue |
| Concentration | 30 U/uL |
| Source / Origin | Escherichia coli K12 |
| Readouts / Downstream Assays | Agarose gel analysis of digested DNA |
For in vitro diagnostic use.
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