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| Product Name | PvuII Rapid Restriction Endonuclease (20 U/uL) |
| Catalog No. | IVDRM-0451 |
| Description | Cutting the palindromic site 5 prime CAG^CTG 3 prime to yield blunt ended fragments, this rapid recombinant endonuclease suits molecular cloning and general DNA fragment preparation, digesting plasmid DNA, PCR products and genomic DNA within 5 to 15 minutes. It is supplied at 20 U/uL and operates in the single shared buffer of the rapid series at 37°C Note that the enzyme cannot be heat inactivated; remove it after digestion by phenol chloroform extraction or column purification. This unit stores at -20°C and ships with ice packs. |
| Intended Use | Molecular cloning and DNA fragment preparation with rapid blunt digestion |
| Principle / Technology | Site specific blunt cleavage at 5 prime CAG^CTG 3 prime within 5 to 15 minutes at 37°C |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; not heat inactivatable, remove enzyme by phenol chloroform extraction or column purification |
| Storage Conditions | -20°C |
| Shelf Life | 2 years |
| Package Specifications | 200 T |
| Key Features | Blunt end output; one shared buffer across the rapid series; large 200 reaction pack |
| Concentration | 20 U/uL |
| Shipping Conditions | Ice packs |
| Readouts / Downstream Assays | Gel electrophoresis of digested DNA |
For in vitro diagnostic use.
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