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| Product Name | PvuI Rapid Restriction Endonuclease (20 U/uL) |
| Catalog No. | IVDRM-0454 |
| Description | Recognising 5 prime CGAT^CG 3 prime, this rapid recombinant endonuclease supports gene cloning, recombinant vector construction and plasmid linearisation, cutting plasmid DNA, PCR products and genomic DNA within 5 to 15 minutes. It is supplied at 20 U/uL and digests in the common rapid series buffer at 37°C, followed by heat inactivation at 80°C for 20 minutes. CpG methylation blocks cleavage at overlapping sites while Dam, Dcm, EcoKI and EcoBI methylation are tolerated, and Ple19I is the documented isoschizomer. |
| Intended Use | Gene cloning, recombinant vector construction and plasmid linearisation with rapid digestion |
| Principle / Technology | Site specific cleavage at 5 prime CGAT^CG 3 prime within 5 to 15 minutes at 37°C |
| Specificity | CpG methylation blocks cleavage; Dam, Dcm, EcoKI and EcoBI have no effect; isoschizomer Ple19I |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 30 T |
| Key Features | One shared buffer for the whole rapid series; activity margin for excess substrate |
| Concentration | 20 U/uL |
| Readouts / Downstream Assays | Digestion profile on agarose gel |
For in vitro diagnostic use.
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