- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | PstI Rapid Restriction Endonuclease (20 U/uL) |
| Catalog No. | IVDRM-0449 |
| Description | This rapid recombinant endonuclease cuts 5 prime CTGCA^G 3 prime to generate a 3 prime overhang and serves molecular cloning with fast digestion of plasmid DNA, PCR products and genomic DNA within 5 to 15 minutes. Supplied at 20 U/uL, it runs in the shared rapid series buffer at 37°C Dam, Dcm, CpG, EcoKI and EcoBI methylation all leave activity unchanged, giving predictable performance across template sources, and BspMAI is the documented isoschizomer. Note that the enzyme cannot be heat inactivated; use phenol chloroform extraction or column purification to remove it after digestion. |
| Intended Use | Molecular cloning with rapid digestion of plasmid DNA, PCR products and genomic DNA |
| Principle / Technology | Site specific cleavage at 5 prime CTGCA^G 3 prime within 5 to 15 minutes at 37°C |
| Specificity | Unaffected by Dam, Dcm, CpG, EcoKI and EcoBI methylation; isoschizomer BspMAI |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; not heat inactivatable, remove enzyme by phenol chloroform extraction or column purification |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 500 T |
| Key Features | Large 500 reaction pack; 3 prime overhang output; complete methylation tolerance |
| Concentration | 20 U/uL |
| Readouts / Downstream Assays | Restriction digest pattern on agarose gel |
For in vitro diagnostic use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
