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| Product Name | PspGI Rapid Restriction Endonuclease (10 U/uL) |
| Catalog No. | IVDRM-0452 |
| Description | Recognising the degenerate site 5 prime CCWGG 3 prime, where W is A or T, this rapid recombinant endonuclease supports gene cloning, vector construction and plasmid linearisation with digestion completed in 5 to 15 minutes. Dcm methylation fully overlapping the site blocks cleavage, a trait exploited in methylation dependent restriction analysis, and the enzyme shares its specificity with a wide isoschizomer panel including EcoRII, MvaI, BstNI, BciT130I, BseBI, AjnI, Psp6I and Bst2UI. Supplied at 10 U/uL, it runs in the common rapid series buffer at 37°C Note that the enzyme cannot be heat inactivated; remove it by phenol chloroform extraction or column purification after digestion. |
| Intended Use | Molecular cloning, vector construction, plasmid linearisation and Dcm methylation dependent restriction analysis |
| Principle / Technology | Site specific cleavage at 5 prime CCWGG 3 prime within 5 to 15 minutes at 37°C |
| Specificity | Dcm methylation fully overlapping the site blocks cleavage; isoschizomers include EcoRII, MvaI, BstNI, BseBI and Psp6I |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; not heat inactivatable, remove enzyme by phenol chloroform extraction or column purification |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 200 T |
| Key Features | Dcm sensitive cutter with broad isoschizomer compatibility; unified buffer across the series |
| Concentration | 10 U/uL |
| Readouts / Downstream Assays | Methylation sensitive restriction pattern on agarose gel |
For in vitro diagnostic use.
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