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| Product Name | Proteinase K Solution for mNGS, Low-Residual (20 mg/mL) |
| Catalog No. | IVDRM-0550 |
| Description | A broad-spectrum serine protease of about 29.3 kDa that cleaves peptide bonds at the carboxyl side of aliphatic and aromatic amino acids, this Proteinase K solution is produced under a low background residue process so that carry-over from background organisms stays minimal and downstream detection accuracy is protected, which suits metagenomic sequencing workflows. Activity is at least 20 mg per mL with specific activity of at least 800 U per mL, active from pH 4.5 to 12.5 with an optimum of 7.0 to 12.0, and digestion runs for one hour at 37 to 65°C with 65°C recommended, inactivated by a PMSF or DFIP inhibitor. Denaturants such as 1% SDS raise its activity, and the common working concentration is 50 to 100 micrograms per millilitre. |
| Intended Use | Low-residual sample preparation for metagenomic sequencing and nuclease removal in nucleic acid workflows |
| Principle / Technology | Broad serine protease digestion of proteins including nucleases under a low background residue process |
| Sample Type | Protein containing lysates for metagenomic sequencing |
| Specificity | Active pH 4.5 to 12.5 with optimum 7.0 to 12.0; digestion at 37 to 65°C with 65°C recommended |
| Reaction Conditions / Protocol | Digest at 37 to 65°C for one hour and inactivate with a PMSF or DFIP inhibitor |
| Storage Conditions | 2 to 8°C; for long term storage keep at -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 1 mL; 5 mL; 100 mL; 1000 mL |
| Key Features | Low background residue process protects downstream detection accuracy; SDS raises activity |
| Purity | Protein concentration at least 20 mg per mL; specific activity at least 800 U per mL; free of RNase and DNase |
| Concentration | 20 mg/mL |
| Readouts / Downstream Assays | Protein digestion completeness in the prepared sample |
For in vitro diagnostic use.
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