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| Product Name | Proteinase K, Lyophilised Powder, RNase-Free and DNase-Free |
| Catalog No. | IVDRM-0544 |
| Description | A broad-spectrum serine protease of about 29 kDa that cleaves peptide bonds at the carboxyl side of aliphatic and aromatic amino acids, Proteinase K is produced from a recombinant yeast strain and is supplied free of RNase and DNase. It stays active across pH 4.0 to 12.0 with an optimum of pH 7.5 to 9.0 and remains stable in urea and SDS, with denaturants such as 1% SDS raising its activity. Common working concentrations are 50 to 100 micrograms per millilitre, chosen according to buffer composition, SDS, urea, pH and temperature. Applications include preparation of chromosomal DNA for pulsed field electrophoresis, protein blotting and removal of nucleases from DNA and RNA preparations. |
| Intended Use | Preparation of chromosomal DNA for pulsed field electrophoresis, protein blotting and nuclease removal in nucleic acid preparation |
| Principle / Technology | Broad serine protease digestion of proteins including nucleases, stable in urea and SDS |
| Sample Type | Protein containing lysates and nucleic acid preparations |
| Reaction Conditions / Protocol | Dissolve the powder and use at 50 to 100 micrograms per millilitre according to the buffer and template |
| Storage Conditions | 2 to 8°C, dry; for longer periods store at -25 to -15°C |
| Shelf Life | 3 years |
| Package Specifications | 100 mg; 1 g; 5 g; 10 g; 100 g |
| Key Features | Active across pH 4.0 to 12.0; stable in urea and SDS; recombinant yeast derived |
| Purity | Free of RNase and DNase |
| Source / Origin | Recombinant yeast strain |
| Readouts / Downstream Assays | Protein digestion completeness in the prepared sample |
For in vitro diagnostic use.
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