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| Product Name | PpuMI Rapid Restriction Endonuclease (10 U/uL) |
| Catalog No. | IVDRM-0456 |
| Description | Recognising the heavily degenerate site 5 prime RG^GWCCY 3 prime, this rapid recombinant endonuclease prepares DNA fragments and supports restriction analysis, cutting plasmid DNA, PCR products and genomic DNA within 5 to 15 minutes. Supplied at 10 U/uL, it operates in the unified rapid series buffer at 37°C and is heat inactivated at 80°C for 20 minutes. Dcm methylation influences cleavage at overlapping sequences, and the isoschizomers PspPPI and Psp5II share the specificity. |
| Intended Use | DNA fragment preparation and restriction endonuclease analysis with rapid degenerate site digestion |
| Principle / Technology | Site specific cleavage at 5 prime RG^GWCCY 3 prime within 5 to 15 minutes at 37°C |
| Specificity | Dcm methylation affects cleavage; isoschizomers PspPPI and Psp5II |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 30 T |
| Key Features | Degenerate six base recognition; single shared buffer across the rapid series |
| Concentration | 10 U/uL |
| Readouts / Downstream Assays | Agarose gel analysis of digested fragments |
For in vitro diagnostic use.
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