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| Product Name | Poly-D-Lysine Coating Solution |
| Catalog No. | ATR-CCR-0031 |
| Description | A synthetic polymer coating solution for tissue culture surfaces that promotes cell attachment through electrostatic interactions. Poly-D-lysine provides a positively charged surface suitable for a broad range of cell types. |
| Intended Use | Enhancement of cell attachment to tissue culture plastic and glass surfaces for primary cells, neuronal cultures, transfected cells, and other cell types that require a positively charged substrate. |
| Principle / Technology | Poly-D-lysine is a synthetic cationic polymer that adsorbs to negatively charged tissue culture surfaces, creating a positively charged monolayer; cells attach through electrostatic interactions between negatively charged cell surface proteoglycans and the poly-D-lysine coating. |
| Detection Method | Cell attachment assessed by phase-contrast microscopy; cell spreading and proliferation monitored using standard culture observation techniques |
| Sample Type | Tissue culture plasticware, glass coverslips, and chamber slides for primary cell culture, neuronal culture, and transfection assays |
| Performance Range / Specifications | Recommended coating concentration: 50–100 μg/mL for most applications; typical volumes: 50 μL/cm² of growth surface; supplied at 1–2 mg/mL sterile solution |
| Sensitivity / LOD | Promotes cell attachment for cell types that otherwise require serum-derived attachment factors or ECM coatings; effective for most primary cell types and many established lines |
| Specificity | Non-specific electrostatic attachment mechanism supports a broad range of cell types without the integrin specificity of ECM proteins; poly-D-lysine (versus poly-L-lysine) is not susceptible to enzymatic degradation |
| Reaction Conditions / Protocol | Dilute poly-D-lysine stock to 50–100 μg/mL in sterile water or PBS, add to culture vessel, incubate at room temperature for 1 hour or overnight at 2–8°C, aspirate excess solution, wash 2–3 times with sterile water, allow to air-dry in a sterile hood, seed cells |
| Components / Formulation | Poly-D-lysine hydrobromide (molecular weight 70,000–150,000) sterile-filtered solution at 1–2 mg/mL in water or PBS |
| Storage Conditions | 2–8°C; can be stored at room temperature for short-term use; do not freeze; protect from repeated temperature changes |
| Shelf Life | 24 months from date of manufacture |
| Package Specifications | 20 mL at 1 mg/mL (sufficient for coating approximately 200–400 cm² of culture surface) |
| Product Form | Sterile, ready-to-use liquid |
| Quality Control | Each lot tested for sterility, endotoxin, and cell attachment-promoting activity using a standard neuronal or transfected cell line adhesion assay; molecular weight range confirmed by viscosity measurement |
| Key Features | Synthetic polymer eliminates risk of biological contaminants present in animal-derived ECM coatings; D-lysine isomer resists cellular protease degradation for stable long-term culture; simple coating protocol with no temperature-sensitive steps |
For research use only, not for clinical use.
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