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| Product Name | PmlI Rapid Restriction Endonuclease (20 U/uL) |
| Catalog No. | IVDRM-0455 |
| Description | Cutting 5 prime CAC^GTG 3 prime to leave a 3 prime overhang, this rapid recombinant endonuclease supports DNA fragment preparation, cloning and digestion based modification work in gene engineering. It digests plasmid DNA, PCR products and genomic DNA within 5 to 15 minutes, is supplied at 20 U/uL, and runs in the shared rapid series buffer at 37°C with heat inactivation at 80°C for 20 minutes. CpG, EcoKI and EcoBI methylation possibly overlapping the site may block cutting, and the isoschizomers include AcvI, Eco72I, PspCI and BbrPI. |
| Intended Use | DNA fragment preparation and cloning for gene engineering workflows |
| Principle / Technology | Site specific cleavage at 5 prime CAC^GTG 3 prime within 5 to 15 minutes at 37°C |
| Specificity | CpG, EcoKI and EcoBI methylation may block cleavage; isoschizomers include AcvI, Eco72I, PspCI and BbrPI |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 100 T |
| Key Features | Single shared buffer across the rapid series; reliable on excess or difficult substrates |
| Concentration | 20 U/uL |
| Readouts / Downstream Assays | Restriction digest pattern on agarose gel |
For in vitro diagnostic use.
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