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| Product Name | PmeI Rapid Restriction Endonuclease (20 U/uL) |
| Catalog No. | IVDRM-0453 |
| Description | Cutting the AT rich site 5 prime TTT^AAAC 3 prime to produce blunt ends, this rapid recombinant endonuclease specialises in plasmid linearisation and vector construction, digesting plasmid DNA, PCR products and genomic DNA within 5 to 15 minutes. Supplied at 20 U/uL, it operates in the unified rapid series buffer at 37°C and is heat inactivated at 80°C for 20 minutes. CpG methylation blocks cleavage at overlapping sequences, and no alternative named isoschizomers are documented. |
| Intended Use | Plasmid linearisation and vector construction with rapid digestion |
| Principle / Technology | Site specific blunt cleavage at 5 prime TTT^AAAC 3 prime within 5 to 15 minutes at 37°C |
| Specificity | CpG methylation blocks cleavage; no alternative named isoschizomers |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 50 T |
| Key Features | AT rich rare cutter for gentle linearisation; single shared buffer across the series |
| Concentration | 20 U/uL |
| Readouts / Downstream Assays | Agarose gel profile of linearised plasmid |
For in vitro diagnostic use.
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