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| Product Name | Plasma Separation Solution, Density Gradient Medium |
| Catalog No. | ATR-SPS-0007 |
| Description | A sterile density gradient medium composed of polysucrose and sodium diatrizoate for the isolation of peripheral blood mononuclear cells and other mononuclear cell populations from whole blood and bone marrow. |
| Intended Use | Separation of lymphocytes and monocytes from whole blood by density gradient centrifugation for immunological assays, cell culture, and flow cytometric analysis. |
| Principle / Technology | Polysucrose and sodium diatrizoate form a solution with density of 1.077 g/mL; during centrifugation, erythrocytes and granulocytes pellet while mononuclear cells form a distinct band at the plasma-gradient interface. |
| Detection Method | Cell count and viability by trypan blue, flow cytometric purity of CD45+ cells |
| Sample Type | Heparinized or citrated whole blood, bone marrow aspirates, cord blood |
| Performance Range / Specifications | PBMC recovery >60% of input; monocyte purity >95%; erythrocyte contamination <5% |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Layer diluted blood onto gradient medium (3:4 ratio); centrifuge at 400 × g for 30 min at 20°C with brake off; harvest PBMC band; wash twice with PBS. |
| Components / Formulation | Polysucrose 5.7% w/v, sodium diatrizoate 9.0% w/v, density 1.077 ± 0.001 g/mL, endotoxin tested |
| Storage Conditions | 15–30°C, protect from light |
| Shelf Life | 36 months in unopened bottle |
| Package Specifications | 100 mL, 500 mL |
| Product Form | Liquid, sterile, ready to use |
| Quality Control | Density verification, sterility, endotoxin <0.12 EU/mL, PBMC recovery validation |
| Key Features | Sterile; endotoxin tested; validated density; standard for PBMC isolation; ready to use |
For research use only, not for clinical use.
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