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| Product Name | Cell Fixation Solution, 4% Paraformaldehyde, Methanol-Free |
| Catalog No. | ATR-SPS-0011 |
| Description | A methanol-free 4% paraformaldehyde fixative in phosphate buffer prepared from electron microscopy grade paraformaldehyde, for the preservation of cellular morphology and antigenicity in flow cytometry and fluorescence microscopy. |
| Intended Use | Fixing cultured cells, dissociated tissue cells, and microbial samples for flow cytometric analysis, immunofluorescence staining, and long-term storage of labeled specimens. |
| Principle / Technology | Formaldehyde cross-links primary amine groups on proteins through methylene bridge formation, preserving cellular architecture; methanol-free preparation prevents membrane permeabilization and lipid extraction during fixation. |
| Detection Method | Flow cytometric forward/side scatter comparison with live cells, IF staining intensity comparison |
| Sample Type | Cultured mammalian cells, dissociated tissue cells, bacteria, yeast |
| Performance Range / Specifications | Fixes >99% of cells; antigen retention >90% for most surface and intracellular antigens after optimized protocol |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Add equal volume of 4% PFA to cell suspension in PBS; incubate 15 min at room temperature in the dark; wash with PBS; resuspend in PBS with 0.1% BSA for storage. |
| Components / Formulation | Paraformaldehyde 4% w/v in PBS pH 7.4, methanol <0.1% |
| Storage Conditions | 2–8°C, protect from light; use within 1 month of opening ampoule; single-use ampoules recommended |
| Shelf Life | 12 months in sealed ampoule |
| Package Specifications | 10 × 1 mL ampoules, 25 mL, 100 mL |
| Product Form | Liquid in sealed ampoule |
| Quality Control | Formaldehyde concentration by titration, methanol content by GC, pH, sterility |
| Key Features | Methanol free preserves membranes; electron microscopy grade; ampoule packaging prevents degradation; validated for flow cytometry |
For research use only, not for clinical use.
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