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| Product Name | Intracellular Staining Permeabilization Buffer, Saponin-Based, 10x |
| Catalog No. | ATR-SPS-0012 |
| Description | A ten-fold concentrated permeabilization buffer containing saponin for the reversible permeabilization of cell membranes, enabling intracellular staining of cytokines, transcription factors, and phosphorylated signaling proteins. |
| Intended Use | Permeabilizing fixed cells for intracellular flow cytometric detection of cytokines (IFN-gamma, IL-2, IL-4, TNF-alpha), transcription factors (FoxP3, T-bet), and phospho-proteins (p-STAT, p-ERK). |
| Principle / Technology | Saponin selectively extracts cholesterol from cell membranes, creating transient pores that permit antibody access to intracellular compartments while preserving overall cellular architecture. |
| Detection Method | Intracellular cytokine detection by flow cytometry, transcription factor staining validation |
| Sample Type | Fixed and permeabilized PBMCs, splenocytes, cultured cell lines, whole blood after RBC lysis |
| Performance Range / Specifications | >90% of cells permeabilized as measured by actin or GAPDH intracellular staining |
| Sensitivity / LOD | N/A |
| Specificity | Saponin-based; reversible permeabilization; compatible with surface epitope staining |
| Reaction Conditions / Protocol | Dilute 1:10; permeabilize fixed cells for 15–20 min at room temperature; use permeabilization buffer for all intracellular staining and wash steps. |
| Components / Formulation | Saponin, HEPES buffer, BSA, sodium azide, in PBS at 10x concentration |
| Storage Conditions | 2–8°C |
| Shelf Life | 12 months |
| Package Specifications | 10 mL concentrate (for 100 mL), 50 mL concentrate |
| Product Form | Liquid concentrate (10x) |
| Quality Control | Permeabilization efficiency, no loss of surface staining during permeabilization, lot-to-lot intensity comparison |
| Key Features | Reversible permeabilization; optimized for intracellular cytokine detection; 10x concentrate for custom dilution; validated with multiple fluorochrome panels |
For research use only, not for clinical use.
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