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| Product Name | Room-Temperature DNA Sample Lysis Kit |
| Catalog No. | ATR-SPS-0033 |
| Description | A rapid sample-preparation system that releases genomic DNA without conventional purification. Optimized lysis chemistry disrupts cellular membranes, while protective and stabilizing components reduce amplification inhibition and support stable lysate storage. |
| Intended Use | Rapid preparation of genomic DNA-containing lysates for direct PCR, probe-based qPCR and SNP genotyping. |
| Principle / Technology | Chemical membrane disruption followed by stabilization of released genomic DNA and neutralization of amplification inhibitors. |
| Detection Method | Direct PCR, probe-based real-time PCR and SNP genotyping. |
| Sample Type | Fresh blood, frozen blood, EDTA-, citrate- or sodium-heparin-anticoagulated blood, tissue homogenate, buccal swab, FTA card and plant tissue. |
| Performance Range / Specifications | Whole-blood lysis in approximately 3 minutes at room temperature. The recommended lysis range for blood is 2-10 µL sample, and 1-10 µL lysate may be used in a 20 µL amplification reaction. |
| Reaction Conditions / Protocol | Combine the sample with the lysis reagents and incubate for 3 minutes at room temperature. Use the resulting lysate directly as amplification template. Mouse-tail material may require a 3-minute treatment at 95°C. |
| Components / Formulation | Lysis buffer and stabilizing buffer containing protein-protective and stabilizing components. |
| Storage Conditions | Store at 2-8°C. |
| Package Specifications | 250 reactions; 1,000 reactions. Reaction counts are based on blood samples. |
| Product Form | Two-component liquid sample lysis kit. |
| Key Features | Three-minute room-temperature workflow; no conventional DNA purification; broad sample compatibility; reduced amplification inhibition; stable prepared lysates. |
| Compatibility | Direct PCR, probe-based qPCR, SNP genotyping and related amplification workflows. |
| Application Notes / Precautions | Use both lysis and stabilizing buffers for optimal inhibitor control and lysate stability. When only lysis buffer is used, reduce template input and limit frozen lysate storage as specified by the protocol. |
For research use only, not for clinical use.
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