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| Product Name | Exosome Isolation Reagent for Plasma |
| Catalog No. | ATR-SPS-0036 |
| Description | A plasma-specific precipitation system for isolating exosomes without ultracentrifugation. Low-speed collection supports intact vesicle morphology, while the included protease can be used to reduce abundant plasma-protein interference when surface-protein analysis is not required. |
| Intended Use | Isolation of plasma-derived exosomes for protein studies, RNA analysis, high-throughput sequencing and cell co-culture research. |
| Principle / Technology | Optional protease pretreatment followed by selective extracellular-vesicle precipitation and low-speed centrifugation. |
| Detection Method | Downstream nanoparticle, protein, RNA and sequencing analyses. |
| Sample Type | Plasma. |
| Performance Range / Specifications | Minimum recommended plasma input: 0.5 mL. Recovered pellets may be resuspended in 1× PBS or directly processed with downstream lysis reagents. |
| Reaction Conditions / Protocol | When appropriate, pretreat plasma with the supplied protease, add the isolation reagent and collect the precipitated vesicles by low-speed centrifugation. |
| Components / Formulation | Plasma exosome isolation reagent and Proteinase K at 20 mg/mL. |
| Storage Conditions | Store the isolation reagent at 2-8°C and Proteinase K at -30 to -15°C. |
| Package Specifications | 10 mL isolation reagent; 2 × 900 µL Proteinase K. |
| Product Form | Liquid precipitation reagent with protease component. |
| Key Features | No ultracentrifuge required; low starting-volume requirement; optional plasma-protein digestion; rapid low-speed workflow; morphology-preserving isolation. |
| Compatibility | Protein analysis, RNA extraction, miRNA analysis, high-throughput sequencing and cell co-culture workflows. |
| Application Notes / Precautions | Proteinase K may digest exosomal surface proteins. Omit the protease pretreatment when downstream analysis depends on intact surface proteins. |
For research use only, not for clinical use.
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